The antiserum was produced against synthesized peptide derived from human CDC25A around the phosphorylation site of Ser124.
Immunogen Range
90-139
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:5000
Specificity
CDC25A (Phospho-Ser124) Antibody detects endogenous levels of CDC25A only when phosphorylated at Ser124.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CDC25A
Database Links
Gene ID: 993 Swiss-Prot #: P30304
OMIM #: 116947 Unigene #: Hs.437705
Target Modification
Phospho
Modification Sites
H:S124 M:S122 R:S124
Application Images
Image 1
Western blot analysis of lysates from 293 cells treated with UV 15', using CDC25A (Phospho-Ser124) Antibody. The lane on the right is blocked with the phospho peptide.