The antiserum was produced against synthesized peptide derived from human CD71/TfR around the phosphorylation site of Ser24.
Immunogen Range
15-64
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity
CD71/TfR (Phospho-Ser24) Antibody detects endogenous levels of CD71/TfR only when phosphorylated at Ser24.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
TFR1
Database Links
Gene ID: 4155 Swiss-Prot #: P02786
OMIM #: 159430 Unigene #: Hs.551713
Target Modification
Phospho
Modification Sites
H:S24 M:S24
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using CD71/TfR (Phospho-Ser24) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30' , using CD71/TfR (Phospho-Ser24) Antibody. The lane on the right is blocked with the phospho peptide.