The antiserum was produced against synthesized peptide derived from human CD44 around the phosphorylation site of Ser706.
Immunogen Range
681-730
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:20000
Specificity
CD44 (Phospho-Ser706) Antibody detects endogenous levels of CD44 only when phosphorylated at Ser706.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CD44
Database Links
Gene ID: 960 Swiss-Prot #: P16070
OMIM #: 107269/172290/609027 Unigene #: Hs.502328
Target Modification
Phospho
Modification Sites
H:S706 M:S742 R:S467
Application Images
Image 1
Western blot analysis of lysates from NIH/3T3 cells treated with PMA 250ng/ml 5', using CD44 (Phospho-Ser706) Antibody. The lane on the right is blocked with the phospho peptide.