Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
| ProductName | CD28(Phospho-Tyr218)Antibody |
| Synonyms | SCFR;Proto-oncogenetyrosine-proteinkinaseKit;c-kit;CD117antigen |
| Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanCD28aroundthephosphorylationsiteofTyr218. |
| ImmunogenRange | 171-220 |
| Clonality | Polyclonal |
| ApplicationDilutions | WB:1:500~1:1000ELISA:1:40000 |
| Specificity | CD28(Phospho-Tyr218)AntibodydetectsendogenouslevelsofCD28onlywhenphosphorylatedatTyr218. |
| Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
| PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
| Storage/StABIlity | Stableat-20°Cforatleast1year. |
| NCBIGeneSymbol | CD28 |
| DatabaseLinks | GeneID: 940 Swiss-Prot#: P10747 OMIM#: 186760 Unigene#: Hs.591629 |
| TargetModification | Phospho |
| ModificationSites | H:Y218M:Y216 |
| ApplicationImages |
| Image1 | WesternblotanalysisoflysatesfromHepG2cellstreatedwithnocodazole1ug/ml16h,usingCD28(Phospho-Tyr218)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |