The antiserum was produced against synthesized peptide derived from human Caspase 9 around the phosphorylation site of Ser196.
Immunogen Range
162-211
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:20000
Specificity
Caspase 9 (Phospho-Ser196) Antibody detects endogenous levels of Caspase 9 only when phosphorylated at Ser196.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CASP9
Database Links
Gene ID: 842 Swiss-Prot #: P55211
OMIM #: 602234 Unigene #: Hs.329502
Target Modification
Phospho
Modification Sites
H:S196
Application Images
Image 1
Western blot analysis of lysates from 293 cells treated with Calyculin 50nM 30', using Caspase 9 (Phospho-Ser196) Antibody. The lane on the right is blocked with the phospho peptide.