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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | PKC alpha (Phospho-Tyr657) Antibody |
| Synonyms | kinase PKC-alpha; KPCA; PKC III; PKC-A; PKC-alpha; PKC-III; PRKACA; PRKCA; Protein kinase C, alpha type |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PKC alpha around the phosphorylation site of Tyr657. |
| Immunogen Range | 623-672 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 ELISA: 1:1000 |
| Specificity | PKC alpha (Phospho-Tyr657) Antibody detects endogenous levels of PKC alpha only when phosphorylated at Tyr657. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | PRKCA |
| Database Links |
Gene ID: 5578 Swiss-Prot #: P17252 OMIM #: 176960 Unigene #: Hs.531704/Hs.708867 |
| Target Modification | Phospho |
| Modification Sites | H:Y657 M:Y657 R:Y657 |
| Application Images | |
| Image 1 |
 Western blot analysis of lysates from COLO205 cells, using PKC alpha (Phospho-Tyr657) Antibody. The lane on the right is blocked with the phospho peptide. |
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