The antiserum was produced against synthesized peptide derived from human Histone H3.3 around the phosphorylation site of Ser31.
Immunogen Range
16-65
Clonality
Polyclonal
Application Dilutions
IF: 1:100~1:500 ELISA: 1:20000
Specificity
Histone H3.3 (Phospho-Ser31) Antibody detects endogenous levels of Histone H3.3 only when phosphorylated at Ser31.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.