The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
Breast Tumor Kinase (Phospho-Tyr447) Antibody
Synonyms
Breast tumor kinase; PTK6; Tyrosine-protein kinase 6; Tyrosine-protein kinase brk
Immunogen
The antiserum was produced against synthesized peptide derived from human Breast Tumor Kinase around the phosphorylation site of Tyr447.
Immunogen Range
402-451
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Specificity
Breast Tumor Kinase (Phospho-Tyr447) Antibody detects endogenous levels of Breast Tumor Kinase only when phosphorylated at Tyr447.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PTK6
Database Links
Gene ID: 5753 Swiss-Prot #: Q13882
OMIM #: 602004 Unigene #: Hs.51133
Target Modification
Phospho
Modification Sites
H:Y447
Application Images
Image 1
Immunofluorescence analysis of NIH/3T3 cells, using Breast Tumor Kinase (Phospho-Tyr447) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using Breast Tumor Kinase (Phospho-Tyr447) Antibody. The lane on the right is blocked with the phospho peptide.