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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | B-RAF (Phospho-Ser602) Antibody |
| Synonyms | B-RAF; B-RAF proto-oncogene serine/threonine-protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase; BRAF; BRAF1; c-RMIL; kinase B-Raf; p94; RAFB1; RMIL; RMIL serine/threonine-protein kinase; v-Raf murine sarcoma viral oncogene homolog B1 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human B-RAF around the phosphorylation site of Ser602. |
| Immunogen Range | 576-625 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000 |
| Specificity | B-RAF (Phospho-Ser602) Antibody detects endogenous levels of B-RAF only when phosphorylated at Ser602. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | BRAF1 |
| Database Links |
Gene ID: 673 Swiss-Prot #: P15056 OMIM #: 114500/115150/164757/211980/605027 Unigene #: Hs.550061 |
| Target Modification | Phospho |
| Modification Sites | H:S602 M:S639 R:S639 |
| Application Images | |
| Image 1 |
 Immunohistochemistry analysis of paraffin-embedded human lymph node, using B-RAF (Phospho-Ser602) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 30', using B-RAF (Phospho-Ser602) Antibody. The lane on the right is blocked with the phospho peptide. |
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