The antiserum was produced against synthesized peptide derived from human BCL-2 around the phosphorylation site of Thr69.
Immunogen Range
46-95
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000
Specificity
BCL-2 (Phospho-Thr69) Antibody detects endogenous levels of BCL-2 only when phosphorylated at Thr69.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
BCL2
Database Links
Gene ID: 10000 Swiss-Prot #: P10415
OMIM #: 151430 Unigene #: Hs.150749
Target Modification
Phospho
Modification Sites
H:T69
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using BCL-2 (Phospho-Thr69) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using BCL-2 (Phospho-Thr69) Antibody. The picture on the right is blocked with the phospho peptide.