The antiserum was produced against synthesized peptide derived from human Bax around the phosphorylation site of Thr167.
Immunogen Range
133-182
Clonality
Polyclonal
Application Dilutions
IF: 1:100~1:500 ELISA: 1:40000
Specificity
Bax (Phospho-Thr167) Antibody detects endogenous levels of Bax only when phosphorylated at Thr167.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
BAX
Database Links
Gene ID: 581 Swiss-Prot #: Q07812
OMIM #: 600040 Unigene #: Hs.433670/Hs.631546
Target Modification
Phospho
Modification Sites
H:T167 M:T167 R:T167
Application Images
Image 1
Immunofluorescence analysis of A549 cells, using Bax (Phospho-Thr167) Antibody. The picture on the right is blocked with the phospho peptide.