Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
| ProductName | Ataxin1(Phospho-Ser776)Antibody |
| Synonyms | ATX1;ATXN1;SCA1;Spinocerebellarataxiatype1protein;Spinocerebellarataxiatype1proteinhomolog |
| Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanAtaxin1aroundthephosphorylationsiteofSer776. |
| ImmunogenRange | 742-791 |
| Clonality | Polyclonal |
| ApplicationDilutions | WB:1:500~1:1000IF:1:100~1:500ELISA:1:10000 |
| Specificity | Ataxin1(Phospho-Ser776)AntibodydetectsendogenouslevelsofAtaxin1onlywhenphosphorylatedatSer776. |
| Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
| PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
| Storage/StABIlity | Stableat-20°Cforatleast1year. |
| NCBIGeneSymbol | ATXN1 |
| DatabaseLinks | GeneID: 6310 Swiss-Prot#: P54253 OMIM#: 164400/601556 Unigene#: Hs.434961 |
| TargetModification | Phospho |
| ModificationSites | H:S776M:S752 |
| ApplicationImages |
| Image1 | ImmunofluorescenceanalysisofNIH/3T3cells,usingAtaxin1(Phospho-Ser776)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
| Image2 | WesternblotanalysisoflysatesfromHepG2cellstreatedwithAdriamycin0.5uM5h,usingAtaxin1(Phospho-Ser776)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |