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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | A-RAF (Phospho-Tyr302) Antibody |
| Synonyms | A-Raf proto-oncogene serine/threonine-protein kinase; A-raf-1; Proto-oncogene Pks; ARAF; ARAF1; PKS; PKS2 |
| Immunogen | The antiserum was produced against synthesized peptide derived from human A-RAF around the phosphorylation site of Tyr302. |
| Immunogen Range | 276-325 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000 |
| Specificity | A-RAF (Phospho-Tyr302) Antibody detects endogenous levels of A-RAF only when phosphorylated at Tyr302. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | ARAF |
| Database Links |
Gene ID: 369 Swiss-Prot #: P10398 OMIM #: 311010 Unigene #: Hs.225936/Hs.446641 |
| Target Modification | Phospho |
| Modification Sites | H:Y302 M:Y300 R:Y300 |
| Application Images | |
| Image 1 |
 Immunofluorescence analysis of HepG2 cells, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using A-RAF (Phospho-Tyr302) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 3 |
 Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30', using A-RAF (Phospho-Tyr302) Antibody. The lane on the right is blocked with the phospho peptide. |
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