The antiserum was produced against synthesized peptide derived from human Actin-pan around the phosphorylation site of Tyr55/53.
Immunogen Range
21-70
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:5000
Specificity
Actin-pan (alpha/gamma) (Phospho-Tyr55/53) Antibody detects endogenous levels of Actin-pan only when phosphorylated at Tyr55/53.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using Actin-pan (alpha/gamma) (Phospho-Tyr55/53) Antibody. The picture on the right is blocked with the phospho peptide.