The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
MSK1 (Phospho-Thr581) Antibody
Synonyms
Nuclear mitogen- and stress-activated protein kinase 1; 90 kDa ribosomal protein S6 kinase 5; RSK-like protein kinase; RSKL
Immunogen
The antiserum was produced against synthesized peptide derived from human MSK1 around the phosphorylation site of Thr581.
Immunogen Range
551-600
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000
Specificity
MSK1 (Phospho-Thr581) Antibody detects endogenous levels of MSK1 only when phosphorylated at Thr581.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RPS6KA5
Database Links
Gene ID: 9252 Swiss-Prot #: O75582
OMIM #: 603607 Unigene #: Hs.510225
Target Modification
Phospho
Modification Sites
H:T581 M:T645
Application Images
Image 1
Immunofluorescence analysis of A549 cells, using MSK1 (Phospho-Thr581) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from RAW264.7 cells treated with UV 5', using MSK1 (Phospho-Thr581) Antibody. The lane on the right is blocked with the phospho peptide.