The antiserum was produced against synthesized peptide derived from human eIF4G around the phosphorylation site of Ser1108.
Immunogen Range
1074-1123
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:40000
Specificity
eIF4G (Phospho-Ser1108) Antibody detects endogenous levels of eIF4G only when phosphorylated at Ser1108.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
EIF4G1
Database Links
Gene ID: 1981 Swiss-Prot #: Q04637
OMIM #: 600495 Unigene #: Hs.433750
Target Modification
Phospho
Modification Sites
H:S1148 M:S1149 R:S1147
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eIF4G (Phospho-Ser1108) Antibody. The picture on the right is blocked with the phospho peptide.