The antiserum was produced against synthesized peptide derived from human CDC25C around the phosphorylation site of Thr48.
Immunogen Range
14-63
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
CDC25C (Phospho-Thr48) Antibody detects endogenous levels of CDC25C only when phosphorylated at Thr48.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CDC25A
Database Links
Gene ID: 995 Swiss-Prot #: P30307
OMIM #: 157680 Unigene #: Hs.656
Target Modification
Phospho
Modification Sites
H:T48
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using CDC25C (Phospho-Thr48) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from RAW264.7 cells, using CDC25C (Phospho-Thr48) Antibody. The lane on the right is blocked with the phospho peptide.