The antiserum was produced against synthesized peptide derived from human ATP-Citrate Lyase around the phosphorylation site of Ser454.
Immunogen Range
420-469
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
ATP-Citrate Lyase (Phospho-Ser454) Antibody detects endogenous levels of ATP-Citrate Lyase only when phosphorylated at Ser454.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ACLY
Database Links
Gene ID: 47 Swiss-Prot #: P53396
OMIM #: 108728 Unigene #: Hs.387567
Target Modification
Phospho
Modification Sites
H:S455 M:S455 R:S454
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using ATP-Citrate Lyase (Phospho-Ser454) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells treated with Calyculin 50nM 30', using ATP-Citrate Lyase (Phospho-Ser454) Antibody. The lane on the right is blocked with the phospho peptide.