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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | Akt (Phospho-Tyr474) Antibody |
| Synonyms | AKT; AKT1 kinase; C-AKT; kinase Akt1; PKB; PKB-alpha; Protein kinase B; RAC; RAC-alpha serine/threonine kinase; RAC-PK-alpha |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Tyr474. |
| Immunogen Range | 431-480 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000 |
| Specificity | Akt (Phospho-Tyr474) Antibody detects endogenous levels of Akt only when phosphorylated at Tyr474. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | AKT1 |
| Database Links |
Gene ID: 207 Swiss-Prot #: P31749 OMIM #: 164730 Unigene #: Hs.525622 |
| Target Modification | Phospho |
| Modification Sites | H:Y474 M:Y474 R:Y474 |
| Application Images | |
| Image 1 |
 Immunohistochemistry analysis of paraffin-embedded human ovary, using Akt (Phospho-Tyr474) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Western blot analysis of lysates from COS7 cells treated with UV 15', using Akt (Phospho-Tyr474) Antibody. The lane on the right is blocked with the phospho peptide. |
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