The antiserum was produced against synthesized peptide derived from human Survivin around the phosphorylation site of Thr117.
Immunogen Range
86-135
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000
Specificity
Survivin (Phospho-Thr117) Antibody detects endogenous levels of Survivin only when phosphorylated at Thr117.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
BIRC5
Database Links
Gene ID: 332 Swiss-Prot #: O15392
OMIM #: 603352 Unigene #: Hs.514527
Target Modification
Phospho
Modification Sites
H:T117 M:T117 R:T117
Application Images
Image 1
Immunofluorescence analysis of A549 cells, using Survivin (Phospho-Thr117) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human brain, using Survivin (Phospho-Thr117) Antibody. The picture on the right is blocked with the phospho peptide.