The antiserum was produced against synthesized peptide derived from human PPAR-gamma around the phosphorylation site of Ser112.
Immunogen Range
78-127
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
Specificity
PPAR-gamma (Phospho-Ser112) Antibody detects endogenous levels of PPAR-gamma only when phosphorylated at Ser112.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of lysates from Jurkat cells treated with Paclitaxel 1uM 24h, using PPAR-gamma (Phospho-Ser112) Antibody. The lane on the right is blocked with the phospho peptide.