The antiserum was produced against synthesized peptide derived from human PAK1/2/3 around the phosphorylation site of Thr423/402/421.
Immunogen Range
391-440
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:5000
Specificity
PAK1/2/3 (Phospho-Thr423/402/421) Antibody detects endogenous levels of PAK1/2/3 only when phosphorylated at Thr423/402/421.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PAK1/2/3 (Phospho-Thr423/402/421) Antibody. The picture on the right is blocked with the phospho peptide.