p70 S6 Kinase (Phospho-Ser418) Antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at Ser418.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
RPS6KB1
Database Links
Gene ID: 6198 Swiss-Prot #: P23443
OMIM #: 608938 Unigene #: Hs.463642
Target Modification
Phospho
Modification Sites
H:S418 M:S441 R:S418
Application Images
Image 1
Immunofluorescence analysis of COS7 cells, using p70 S6 Kinase (Phospho-Ser418) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 S6 Kinase (Phospho-Ser418) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3
Western blot analysis of lysates from HT29 cells treated with serum 20% 15', using p70 S6 Kinase (Phospho-Ser418) Antibody. The lane on the right is blocked with the phospho peptide.