Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | LAT(Phospho-Tyr191)Antibody |
Synonyms | 36kDaphospho-tyrosineadaptorprotein;LinkerforactivationofTcells;p36-38;pp36 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanLATaroundthephosphorylationsiteofTyr191. |
ImmunogenRange | 191-240 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:1000 |
Specificity | LAT(Phospho-Tyr191)AntibodydetectsendogenouslevelsofLATonlywhenphosphorylatedatTyr191. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | LAT |
DatabaseLinks | GeneID: 27040 Swiss-Prot#: O43561 OMIM#: 602354 Unigene#: Hs.632179 |
TargetModification | Phospho |
ModificationSites | H:Y191M:Y195R:Y195 |
ApplicationImages |
Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumancoloncarcinoma,usingLAT(Phospho-Tyr191)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromNIH/3T3cells,usingLAT(Phospho-Tyr191)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |