The antiserum was produced against synthesized peptide derived from human FAK around the phosphorylation site of Tyr407.
Immunogen Range
373-422
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
FAK (Phospho-Tyr407) Antibody detects endogenous levels of FAK only when phosphorylated at Tyr407.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
PTK2
Database Links
Gene ID: 5747 Swiss-Prot #: Q05397
OMIM #: 600758 Unigene #: Hs.395482
Target Modification
Phospho
Modification Sites
H:Y407 M:Y438 R:Y407
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using FAK (Phospho-Tyr407) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using FAK (Phospho-Tyr407) Antibody. The lane on the right is blocked with the phospho peptide.