The antiserum was produced against synthesized peptide derived from human ERF around the phosphorylation site of Thr526.
Immunogen Range
492-541
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000
Specificity
ERF (Phospho-Thr526) Antibody detects endogenous levels of ERF only when phosphorylated at Thr526.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ERF
Database Links
Gene ID: 2077 Swiss-Prot #: P50548
OMIM #: 611888 Unigene #: Hs.655969
Target Modification
Phospho
Modification Sites
H:T56 M:T529
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using ERF (Phospho-Thr526) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using ERF (Phospho-Thr526) Antibody. The picture on the right is blocked with the phospho peptide.