The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
CDK1/CDC2 (Phospho-Thr14) Antibody
Synonyms
Cell division protein kinase 2; kinase Cdk2; p33 protein kinase
Immunogen
The antiserum was produced against synthesized peptide derived from human CDK1/CDC2 around the phosphorylation site of Thr14.
Immunogen Range
Jan-50
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000
Specificity
CDK1/CDC2 (Phospho-Thr14) Antibody detects endogenous levels of CDK1/CDC2 only when phosphorylated at Thr14.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CDK2
Database Links
Gene ID: 1017 Swiss-Prot #: P24941
OMIM #: 116953 Unigene #: Hs.19192/Hs.689624
Target Modification
Phospho
Modification Sites
H:T14 M:T14 R:T14
Application Images
Image 1
Immunofluorescence analysis of COS7 cells, using CDK1/CDC2 (Phospho-Thr14) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HepG2 cells treated with Forskolin 40nM 30', using CDK1/CDC2 (Phospho-Thr14) Antibody. The lane on the right is blocked with the phospho peptide.