The antiserum was produced against synthesized peptide derived from human BCL-2 around the phosphorylation site of Ser87.
Immunogen Range
61-110
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
BCL-2 (Phospho-Ser87) Antibody detects endogenous levels of BCL-2 only when phosphorylated at Ser87.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
BCL2
Database Links
Gene ID: 10000 Swiss-Prot #: P10415
OMIM #: 151430 Unigene #: Hs.150749
Target Modification
Phospho
Modification Sites
H:S87 M:S84 R:S84
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using BCL-2 (Phospho-Ser87) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HeLa cells treated with nocodazole 1ug/ml 18h, using BCL-2 (Phospho-Ser87) Antibody. The lane on the right is blocked with the phospho peptide.