The antiserum was produced against synthesized peptide derived from human ADD1 around the phosphorylation site of Thr445.
Immunogen Range
411-460
Clonality
Polyclonal
Application Dilutions
IHC: 1:50~1:100 ELISA: 1:1000
Specificity
ADD1 (Phospho-Thr445) Antibody detects endogenous levels of ADD1 only when phosphorylated at Thr445.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ADD1
Database Links
Gene ID: 118 Swiss-Prot #: P35611
OMIM #: 102680 Unigene #: Hs.183706/Hs.701490
Target Modification
Phospho
Modification Sites
H:T445 M:T445 R:T445
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ADD1 (Phospho-Thr445) Antibody. The picture on the right is blocked with the phospho peptide.