The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
GRF-1 (Phospho-Tyr1105) Antibody
Synonyms
GAP-associated protein p190, GRF1, GRLF, GRLF1, Glucocorticoid receptor DNA binding factor 1, Glucocorticoid receptor repression factor 1, KIAA1722, Rho GAP p190A, p190-A
Immunogen
The antiserum was produced against synthesized peptide derived from human GRF-1 around the phosphorylation site of Tyr1105.
Immunogen Range
1071-1120
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
GRF-1 (Phospho-Tyr1105) Antibody detects endogenous levels of GRF-1 only when phosphorylated at Tyr1105.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
GRLF1
Database Links
Gene ID: 2909 Swiss-Prot #: Q9NRY4
OMIM #: 605277 Unigene #: Hs.509447
Target Modification
Phospho
Modification Sites
H:Y1105 M:Y943 R:Y1105
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRF-1 (Phospho-Tyr1105) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using GRF-1 (Phospho-Tyr1105) Antibody. The lane on the right is blocked with the phospho peptide.