The antiserum was produced against synthesized peptide derived from human Dynamin-1 around the phosphorylation site of Ser774.
Immunogen Range
740-789
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
Dynamin-1 (Phospho-Ser774) Antibody detects endogenous levels of Dynamin-1 only when phosphorylated at Ser774.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
DNM1
Database Links
Gene ID: 1759 Swiss-Prot #: Q05193
OMIM #: 602377 Unigene #: Hs.522413
Target Modification
Phospho
Modification Sites
H:S774 M:S770 R:S774
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human brain, using Dynamin-1 (Phospho-Ser774) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from mouse brain, using Dynamin-1 (Phospho-Ser774) Antibody. The lane on the right is blocked with the phospho peptide.