The antiserum was produced against synthesized peptide derived from human Abl around the phosphorylation site of Tyr412.
Immunogen Range
361-410
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
Abl (Phospho-Tyr412) Antibody detects endogenous levels of Abl only when phosphorylated at Tyr412.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Abl (Phospho-Tyr412) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from COS7 cells treated with Adriamycin 0.5ug/ml 24h, using Abl (Phospho-Tyr412) Antibody. The lane on the right is blocked with the phospho peptide.