The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
SRF (Phospho-Ser99) Antibody
Synonyms
serum response factor
Immunogen
The antiserum was produced against synthesized peptide derived from human SRF around the phosphorylation site of Ser99.
Immunogen Range
71-120
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Specificity
SRF (Phospho-Ser99) Antibody detects endogenous levels of SRF only when phosphorylated at Ser99.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
SRF
Database Links
Gene ID: 6722 Swiss-Prot #: P11831
OMIM #: 600589 Unigene #: Hs.520140
Target Modification
Phospho
Modification Sites
H:S103 M:S99 R:S99
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using SRF (Phospho-Ser99) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from LOVO cells treated with Serum 10% 15', using SRF (Phospho-Ser99) Antibody. The lane on the right is blocked with the phospho peptide.