Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | HSL(Phospho-Ser855/554)Antibody |
Synonyms | Hormonesensitivelipase;LIPE;LIPS |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanHSLaroundthephosphorylationsiteofSer855/554. |
ImmunogenRange | 520-569 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:20000 |
Specificity | HSL(Phospho-Ser855/554)AntibodydetectsendogenouslevelsofHSLonlywhenphosphorylatedatSer855/554. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | LIPE |
DatabaseLinks | GeneID: 3991 Swiss-Prot#: Q05469 OMIM#: 151750 Unigene#: Hs.656980 |
TargetModification | Phospho |
ModificationSites | H:S855M:S559R:S865 |
ApplicationImages |
Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumancoloncarcinoma,usingHSL(Phospho-Ser855/554)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromHeLacellstreatedwithAdriamycin0.5ng/ml24h,usingHSL(Phospho-Ser855/554)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |