Note:
Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures |
ProductName | CyclinB1(Phospho-Ser126)Antibody |
Synonyms | CCN-2;CCNB;CCNB1;CCNB1-RS1;CYCB;CYCB1;G2/mitotic-specificcyclinB1 |
Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanCyclinB1aroundthephosphorylationsiteofSer126. |
ImmunogenRange | 91-140 |
Clonality | Polyclonal |
ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100ELISA:1:5000 |
Specificity | CyclinB1(Phospho-Ser126)AntibodydetectsendogenouslevelsofCyclinB1onlywhenphosphorylatedatSer126. |
Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
Storage/StABIlity | Stableat-20°Cforatleast1year. |
NCBIGeneSymbol | CCNB1 |
DatabaseLinks | GeneID: 891 Swiss-Prot#: P14635 OMIM#: 123836 Unigene#: Hs.23960 |
TargetModification | Phospho |
ModificationSites | H:S126M:S123R:S116 |
ApplicationImages |
Image1 | Immunohistochemistryanalysisofparaffin-embeddedhumanlungcarcinoma,usingCyclinB1(Phospho-Ser126)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
Image2 | WesternblotanalysisoflysatesfromNIH/3T3cellstreatedwithEGF200ng/ml15",usingCyclinB1(Phospho-Ser126)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |