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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | CrkII (Phospho-Tyr221) Antibody |
| Synonyms | Adapter molecule crk; CRKO; Crk-II; P38; Proto-oncogene C-crk |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CrkII around the phosphorylation site of Tyr221. |
| Immunogen Range | 187-236 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:40000 |
| Specificity | CrkII (Phospho-Tyr221) Antibody detects endogenous levels of CrkII only when phosphorylated at Tyr221. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | CRK |
| Database Links |
Gene ID: 1398 Swiss-Prot #: P46108 OMIM #: 164762 Unigene #: Hs.638121 |
| Target Modification | Phospho |
| Modification Sites | H:Y221 M:Y221 R:Y221 |
| Application Images | |
| Image 1 |
 Immunofluorescence analysis of HUVEC cells, using CrkII (Phospho-Tyr221) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Western blot analysis of lysates from COS7 cells, using CrkII (Phospho-Tyr221) Antibody. The lane on the right is blocked with the phospho peptide. |
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