The product is for research use only, not for use in diagnostic or therapeutic procedures
Product Name
C/EBP-alpha (Phospho-Ser21) Antibody
Synonyms
C/EBP alpha; CCAAT/enhancer binding protein (C/EBP), alpha; CCAAT/enhancer binding protein alpha; CEBPA
Immunogen
The antiserum was produced against synthesized peptide derived from human C/EBP-alpha around the phosphorylation site of Ser21.
Immunogen Range
Jun-55
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
C/EBP-alpha (Phospho-Ser21) Antibody detects endogenous levels of C/EBP-alpha only when phosphorylated at Ser21.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
CEBPA
Database Links
Gene ID: 1050 Swiss-Prot #: P49715
OMIM #: 116897 Unigene #: Hs.699463
Target Modification
Phospho
Modification Sites
H:S21 M:S21 R:S21
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using C/EBP-alpha (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 5', using C/EBP-alpha (Phospho-Ser21) Antibody. The lane on the right is blocked with the phospho peptide.