The antiserum was produced against synthesized peptide derived from human BTK around the phosphorylation site of Tyr551.
Immunogen Range
516-565
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 ELISA: 1:40000
Specificity
BTK (Phospho-Tyr551) Antibody detects endogenous levels of BTK only when phosphorylated at Tyr551.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
BTK
Database Links
Gene ID: 695 Swiss-Prot #: Q06187
OMIM #: 300300/300755/307200 Unigene #: Hs.159494
Target Modification
Phospho
Modification Sites
H:Y551 M:Y551 R:Y552
Application Images
Image 1
Western blot analysis of lysates from HeLa cells treated with H2O2 100uM 30', using BTK (Phospho-Tyr551) Antibody. The lane on the right is blocked with the phospho peptide.