The antiserum was produced against synthesized peptide derived from human Acetyl-CoA Carboxylase around the phosphorylation site of Ser80.
Immunogen Range
46-95
Clonality
Polyclonal
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
Specificity
Acetyl-CoA Carboxylase (Phospho-Ser80) Antibody detects endogenous levels of Acetyl-CoA Carboxylase only when phosphorylated at Ser80.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
ACACA
Database Links
Gene ID: 31 Swiss-Prot #: Q13085
OMIM #: 200350 Unigene #: Hs.160556
Target Modification
Phospho
Modification Sites
H:S80 M:S79 R:S79
Application Images
Image 1
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Acetyl-CoA Carboxylase (Phospho-Ser80) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 5', using Acetyl-CoA Carboxylase (Phospho-Ser80) Antibody. The lane on the right is blocked with the phospho peptide.