IkappaB-epsilon (Phospho-Ser22) Antibody detects endogenous levels of IkappaB-epsilon only when phosphorylated at Ser22.
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability
Stable at -20°C for at least 1 year.
NCBI Gene Symbol
NFKBIE
Database Links
Gene ID: 4794 Swiss-Prot #: O00221
OMIM #: 604548 Unigene #: Hs.458276
Target Modification
Phospho
Modification Sites
H:S22 M:S22 R:S22
Application Images
Image 1
Immunofluorescence analysis of HeLa cells, using IkappaB-epsilon (Phospho-Ser22) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-epsilon (Phospho-Ser22) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3
Western blot analysis of lysates from Jurkat cells treated with TNF-a 20ng/ml 30', using IkappaB-epsilon (Phospho-Ser22) Antibody. The lane on the right is blocked with the phospho peptide.