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| Note: The product is for research use only, not for use in diagnostic or therapeutic procedures |
|
| Product Name | HSP90B (Phospho-Ser254) Antibody |
| Synonyms | HS90B; HSP 84; HSP90-beta; HSP90AB1; HSPC2; HSPCB; Heat shock protein HSP 90-beta |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser254. |
| Immunogen Range | 226-275 |
| Clonality | Polyclonal |
| Application Dilutions | WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000 |
| Specificity | HSP90B (Phospho-Ser254) Antibody detects endogenous levels of HSP90B only when phosphorylated at Ser254. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage/Stability | Stable at -20°C for at least 1 year. |
| NCBI Gene Symbol | HSP90AB1 |
| Database Links |
Gene ID: 3326 Swiss-Prot #: P08238 OMIM #: 140572 Unigene #: Hs.509736/Hs.689683 |
| Target Modification | Phospho |
| Modification Sites | H:S254 M:S254 R:S254 |
| Application Images | |
| Image 1 |
 Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using HSP90B (Phospho-Ser254) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 2 |
 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSP90B (Phospho-Ser254) Antibody. The picture on the right is blocked with the phospho peptide. |
| Image 3 |
 Western blot analysis of lysates from HeLa cells treated with TNF-a 10ng/ml 30', using HSP90B (Phospho-Ser254) Antibody. The lane on the right is blocked with the phospho peptide. |
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