Properties:
| RelatedCategories | AnalyticalColumn,5μm,Analytical/Chromatography,DiscoveryHPLCColumns,DiscoveryHSF5,HPLC, |
| feature | endcapped |
| extentoflabeling | 12%CarbonloADIng |
| parameter | ≤70°Ctemp.range |
| application(s) | HPLC:suitable |
| L×I.D. | 10 cm×2.1 mm |
| surfacecoverage | 4 μmol/m2 |
| impurities | <10 ppmmetals |
| matrix | silicagel,highpurity,sphericalparticleplatform |
| matrixactivegroup | PFP(pentafluorophenyl)phase |
| particlesize | 5 μm |
| poresize | 120 ? |
| surfacearea | 300 m2/g |
| operatingpHrange | 2-8 |
| suitABIlity | suitableforL43perUSP |
| modeofchromatography | Hydrophilicinteraction(HILIC) |
| | Reversedphase |
Description:
FeaturesandBenefits
• Uniqueselectivity
• SimilarretentiontoC18(sometimesrequiresstrongermobilephase)
• Excellentpeakshape
• Stable,low-bleedLC-MSseparations
• Scalableseparationsfrom3to10μmparticlesizes
Generaldescription
GuidelinesfortransferringaC18methodtoDiscovery®HSF5:
Generally,basesareretainedlongerontheHSF5thanonaC18.IncreasingtheorganiccontentofaC18separation5to10percentwillgenerallyprovidesimilarretentiononanHSF5.Resultswithothercompoundsarehighlyvariable.However,itisgenerallytruethatsoluteswithlog?Po/wvalueslessthan2.5willberetainedlongeronHSF5comparedtoaC18.Thedegreeofdifferenceishighlysolutedependent.
TheDiscovery®HSF5bondedphaseprovidesreversed-phaseseparationsthataredistinctlydifferentfromC18columns.However,compoundswillgenerallyelutewithinthesameretentiontimewindow,makingmostC18methodseasilytransferable.
Recommendedproducts
DiscoverLiChropurreagentsidealforHPLCorLC-MSanalysis
LegalInformation
DiscoveryisaregisteredtrademarkofSigma-AldrichCo.LLC
Safety:
PersonalProtectiveEquipment
Eyeshields,Gloves,typeN95(US),typeP1(EN143)respiratorfilter
RIDADR
NONHforallmodesoftransport
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