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    當前位置 : Millipore >>> Millipore/506123 | Anti-p38 MAP Kinase (341-360) Rabbit pAb/506123/
    Millipore/506123 | Anti-p38 MAP Kinase (341-360) Rabbit pAb/506123/
    • Millipore/506123 | Anti-p38 MAP Kinase (341-360) Rabbit pAb/506123/

    Millipore/506123 | Anti-p38 MAP Kinase (341-360) Rabbit pAb/506123/

    價格: ¥4440.00 市場價: 7400.00

    貨號: 506123
    品牌: Millipore
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      • Description
        OverviewRecognizesthe~38kDap38MAPKprotein.
      • AntibodyTargetGeneSymbol:MAPK14
      • TargetSynonym:CRK1,CSBP,CSBP1,CSBP2,CSPB1,EXIP,Hog,MAPKp38,MGC102436,MGC105413,MXI2,P38,P38KINASE,P38MapKinase,p38Mapkalpha,P38-ALPHA,p38-RK,p38/Hog1,p38/Mpk2,P38/RK,p38a,p38Hog,p38MAPK,PRKM14,PRKM15,RK,SAPK2A
      • EntrezGeneName:mitogen-activatedproteinkinase14
      • HuEntrezID:1432
      • MuEntrezID:26416
      • RatEntrezID:81649
      • CatalogueNumber506123
        BrandFamilyCalbiochem®
        ApplicationData
        Detectionofp38MAPkinasebyimmunoblotting.Samples:C6cells(lanes1and2)treated(lane1)oruntreated(lane2)withanisomycinandNIH/3T3cells(lanes3and4)treated(lane3)oruntreated(lane4)withUV.Primaryantibody:Anti-p38MAPKinase(341-360)RabbitpAb(Cat.No.506123)(1:1000).Detection:chemiluminescence.
        ProductInformation
        FormLiquid
        FormulationIn150mMNaCl,10mMHEPES,50%glycerol,0.01%BSA,pH7.5.
        PreservativeNone
        StorageandShippingInformation
        ShipCodeBlueIceOnly
        ToxicityStandardHandling
        Storage-20°C
        Avoidfreeze/thawAvoidfreeze/thaw
        DonotfreezeOktofreeze
        SpecialInstructionsFollowinginitialthaw,aliquotandfreeze(-20°C).
        Applications
        KeyApplicationsFlowCytometry
        Immunoblotting(WesternBlotting)
        ParaffinSections
        ApplicationNotesFlowCytometry(1:25)
        Immunoblotting(1:1000)
        ParaffinSections(1:50,heatpretreatmentrequired,seecomments)
        ApplicationCommentsPretreatparaffinsectionsbyheatingtissuein10mMcitratebuffer,pH6.0for1minathighpowerfollowedby9minatmediumpower;keeptheslidesfullyimmersedandmaintainthetemperatureatorjustbelowboiling;cooltheslidesfor20minatroomtemperaturepriortostaining.Variablesassociatedwithassayconditionswilldictatetheproperworkingdilution.

        RecommendedProtocolforImmunoblotting

        SolutionsandReagents
        ?TransferBuffer:25mMTrisbase,0.2Mglycine,20%methanol,pH8.5.
        ?SDSSampleBuffer:62.5mMTris-HCl,pH6.8,2%SDS,10%glycerol,50mMDTT,0.1%bromophenolblue.
        ?10XTBS(Tris-bufferedsaline):Toprepare1liter,24.2gTrisbase,80gNaCl,adjustpHto7.6withHCl.Dilute1:10foruse.
        ?BlockingBuffer:1XTBS,0.1%Tween?-20detergentwith5%non-fatdrymilk.
        ?PrimaryAntibodyDilutionBuffer:1XTBS,0.1%Tween?-20detergentwith5%BSA
        ?WashBuffer(TBST):1XTBS,0.1%Tween?-20detergent

        BlottingMembrane
        NitrocelluloseorPVDFmembranesmaybeused.

        ProteinBlotting
        1.Lysecellsbyadding100mlSDSSampleBufferandimmediatelyscrapethecellsofftheplateandtransfertheextracttoamicrofugetube.Keeponice.
        2.Sonicatefor2stoshearDNAandreducesampleviscosity.
        3.Heatsampleto95-100°Cfor5min.Coolonice.
        4.Microcentrifugefor5min.
        5.Load20mlontoSDS-PAGEgel(10cmx10cm).
        6.Electrotransfertonitrocellulosemembrane.

        Ascontrols,werecommendusing15mlofphosphorylatedandnonphosphorylatedC-6gliomacellextracts.

        MembraneBlocking,GelandAntibodyIncubations
        1.Aftertransfer,washmembranewith25mlTBSfor5minatroomtemperature.
        2.Incubatemembranein25mlofBlockingBufferfor1-3hatroomtemperatureorovernightat4°C.
        3.Wash3timesfor5mineachwith15mlTBST.
        4.Incubatemembraneandprimaryantibody(attheappropriatedilution)in10mlPrimaryAntibodyDilutionBufferwithgentleagitationovernightat4°C.
        5.Wash3timesfor5mineachwith15mlTBST.
        6.Incubatemembranewithconjugatedsecondaryantibodyattheappropriatedilutionin10mlBlockingBufferwithgentleagitationfor1hatroomtemperature.
        7.Washmembraneasinstep5.

        DetectionofProteins
        Chemiluminescence.
        BIOLOGicalInformation
        Immunogenasyntheticpeptide(TYDEYISFVPPPLDQEEMES)correspondingtoaminoacids341-360ofhumanp38MAPkinase
        ImmunogenHuman
        HostRabbit
        IsotypeIgG
        SpeciesReactivity
        • Human
        • Mouse
        • Rat
        AntibodyTypePolyclonalAntibody
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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