Modularthermalcyclerplatform,includesC1000Touch™ThermalCyclerChassis,CFX384™OpticalReactionModule,CFXMaestro™Software,qbase+Softwarelicense,cables,reagents,consumablesDescription:
UsetheCFX384TouchReal-TimePCRDetectionSystemforprecise,reproducIBLereal-timePCR.ThesystemiscomposedoftheCFX384™OpticalReactionModuleandtheC1000TouchThermalCyclerChassis,SYBR®GreenSupermix,384-wellPCRplatesandplateseals.
ThemoduleisamemberofthefamilyofinterchangeablereactionmodulesthatcanbeusedwiththeC1000TouchThermalCyclerChassisforPCRandreal-timePCR.Allmodulescanbequicklyinsertedandremovedfromthecyclerchassiswithouttools.
- Detectionofupto4targetsperwell,plusachanneldedicatedtosingleplexFRET
- Protocolautowriterthatgeneratesanoptimalprotocolforyourreactioncomponents
- ThermalgrADIentfeaturethatidentifiesoptimalannealingtemperatureinasinglerun
- CFXMaestroSoftwareandqbase+Softwareforreliablevalidation,dataanalysis,andexportofRDMLfilesforconformancewithMIQEguidelines
TheCFX384TouchReal-TimePCRDetectionSystemhassixindependentlycontrolledthermalelectricunitsprovidingeven,preciselycontrolledtemperaturesatalltimesduringtherun,includingramping.Thereducedmassofthehoneycombblockprovidesfastrampingandreducedsettlingtime(timetoachievethermaluniformity).
Theopticalsystem,withfivefilteredLEDsandfivefilteredphotodiodes,collectsdatafromallwells,detectinguptofourtargetsperwell.Forsingle-colorFAMandSYBRGreenI,thefastscanoptionreadssingle-channelfluorescenceinall384wellsinjust8seconds.AchannelwithanLEDfilter–photodiodecombinationisdedicatedtoFRETsingleplexexperiments.
Thelargetouch-screeninterfacehasintuitivegraphicalprogramminganddisplaysdatatracesinrealtime.Theprogrammablethermalgradientfeaturecantesteighttemperaturesatoncetoquicklydeterminetheoptimalannealingtemperature,andtheprotocolautowriterquicklyoptimizesreactions,generatinganoptimalprotocolbasedonyourpolymerase,primers,andproductlength.
- CFX384OpticalReactionModule
- C1000TouchThermalCyclerChassis
- USBcable
- CFXMaestroSoftware
- Instructionmanual
- qbase+Softwarelicense
- SsoAdvanced™UniversalSYBR®GreenSupermix(1725270)
- Hard-Shell®Thin-Wall384-WellSkirtedPCRPlates(HSP3805)
- Microseal®"B"AdhesiveSeals(MSB1001)
ThefollowingmodulescanbeusedwiththeC1000TouchThermalCyclerChassis:
| ReactionModules | CatalogNumber |
| | Module | ModulewithStarterPackage* |
| ForPCR | | |
| Dual48/48FastReactionModule | 1840148 | — |
96-WellFastReactonModule
| 1840196 | — |
| 96–DeepWellReactionModule | 1840197 | — |
| 384-WellReactionModule | 1840138 | — |
| ForReal-TimePCR | | |
| CFX96™OpticalReactionModule | 1845097 | 1845096 |
| CFX96DeepWellOpticalReactionModule | 1844095 | 1844096 |
| CFX384™OpticalReactionModule | 1845385 | 1845384 |
*StarterpackagecontainsSsoAdvancedUniversalSYBRGreenSupermix(1725270),Microseal"B"AdhesiveSeals(MSB1001),andHard-ShellLow-ProfileThin-Wall96-WellSkirtedPCRPlates(HSP9601)orHard-ShellThin-Wall384-WellSkirtedPCRPlates(HSP3805).
TheCFX384TouchReal-TimePCRDetectionSystemcanbeconfiguredwithotherPCRinstruments,allowingstand-aloneorsoftwarecontrol.InstrumentpermutationsallowuptofourdifferentCFXConnect™,CFX96,orCFX384SystemstobecontrolledbyonePC.
CombineanytwoCFXReal-TimePCRDetectionSystemswiththeCFXAutomationSystemII,ourroboticplatehandler,tocreateahigh-throughput,automatedreal-timePCRsystemwithacompactfootprint.
- Bio-RadExpertCareServiceoffersseveralserviceplanstokeepyourCFXSystemsrunning,includingensuringthatyourreal-timePCRsystemsarefunctioningoptimally
- CertifysysteminstallationandvalidatesystemperformancewithCFXSystemIQ/OQService
- VerifytheperformanceofyourCFXSystemswithThermalValidationServicesandtheCFXQualificationPlates
- C1000TouchThermalCyclerChassis(1841100)
- PX1™PCRPlateSealer
- PCRplasticsoptimizedforCFXReal-TimePCRDetectionSystems:
- Hard-Shell384-WellSkirtedPCRPlates
- Microseal"B"AdhesiveSeals(MSB1001)
- OpticallyClearHeatSeals(1814030)
- CFXMaestroSoftware,SecurityEdition,forcompliancewithU.S.FDA21CFRPart11regulations
- CFXQualificationPlateforperformanceverification,384-well (1845099)
- Real-TimePCRDetectionSystems
- PCRReagentsandqPCRReagents
- PCRPlasticConsumables
- PCRSeals
- PCRAnalysisSoftware
- CFX384TouchReal-TimePCRDetectionSystemSpecificationsSheet
- CFX384TouchReal-TimePCRSystemInstallationQuickGuide
- CFX96Touch,CFX96TouchDeepWell,andCFX384TouchReal-TimePCRDetectionSystemsInstructionManual
- CFXQualificationPlate—384-WellPlateFormatProductInsert
- Real-TimePCRApplicationsGuide
- Real-TimePCRDetectionSystemsSelectionChart
- qPCR/Real-TimePCR
- qPCRInstrumentation
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