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    當前位置 ： Millipore >>> Millipore/17-650 | ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set/17-650/25 assays25 assays per
    Millipore/17-650 | ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set/17-650/25 assays25 assays per
    • Millipore/17-650 | ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set/17-650/25 assays25 assays per

    Millipore/17-650 | ChIPAb+ Ubiquityl-Histone H2B - ChIP Validated Antibody and Primer Set/17-650/25 assays25 assays per

    價格: ￥4680.00 市場價: 7800.00
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    貨號: 17-650
    品牌: Millipore
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      • Description
        CatalogueNumber17-650
        BrandFamilyUpstate
        TradeName
        • ChIPAb+
        • Upstate
        DescriptionChIPAb+Ubiquityl-HistoneH2B-ChIPValidatedAntibodyandPrimerSet
        OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
        TheChIPAb+Ubiquityl-HistoneH2B,(clone56)setincludestheUbiquityl-HistoneH2B,(clone56)antibody,anegativecontrolantibody(purifiedMouseIgG),andqPCRprimerswhichamplifya213bpregionwithinthecodingregionofthehumanGAPDHgene.
        Theubiquityl-HistoneH2Bandnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofUbiquityl-HistoneH2Bassociatedchromatin.
        AlternateNames
        • H2BUb
        • HistoneH2B(ubiquityl)
        • H2Bhistonefamily,memberL
        • H2B.1A
        • histone1
        • H2bc
        • histonecluster1
        • H2bc
        ProductInformation
        FormatProteinGPurified
        Control
        • IncludesnegativecontrolmouseIgGantibodyandprimersspecificforhumanGAPDHcodingregion.
        PresentationAnti-ubiquityl-HistoneH2B,clone56(mousemonoclonalIgG).Onevialcontaining25μgofproteinGpurifiedantibodyin25μLPBScontaining0.05%sodiumazide.Storeat-20°C.

        NormalMouseIgG.Onevialcontaining25μgpurifiedmouseIgGin25μLstoragebuffercontaining0.1%sodiumazide.Storeat-20°C.

        ChIPPrimersGAPDHCodingRegion.Onevialcontaining75μLof5μMofeachprimerspecificforthecodingregionofhumanGAPDH.Storeat-20°C.
        FOR:GGCTCCCACCTTTCTCATCC
        REV:GGCCATCCACAGTCTTCTGG
        StorageandShippingInformation
        StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
        Applications
        ApplicationThisChIPAb+Ubiquityl-HistoneH2B-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
        KeyApplications
        • WesternBlotting
        • Immunofluorescence
        • ChromatinImmunoprecipitation(ChIP)
        ApplicationNotesChromatinImmunoprecipitation:
        SonicatedchromatinpreparedfromHeLaS3cells(1X106cellequivalentsperIP)wassubjectedtochromatinimmunoprecipitationusing1μgofeitheranormalmouseIgGorAnti-Ubiquityl-HistoneH2BantibodyandtheMagnaChIPGKit(Cat.#17-611).
        Successfulimmunoprecipitationofubiquityl-HistoneH2BassociatedDNAfragmentswasverifiedbyqPCRusingGAPDHpromoter(negative)andGAPDHcoding(positive)Primers(Pleaseseefigures).DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.
        PleaserefertotheEZ-MagnaGChIP?(Cat.#17-409)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails.

        WesternBlotAnalysis:
        HeLacelllysatewasresolvedbyelectrophoresis,transferredtoPVDFandprobedwithanti-Ubiquityl-histoneH2Batadilutionof1:4000(Pleaseseefigures).


        BiologicalInformation
        ImmunogenImmunogenusedwasabranchedpeptidecorrespondingtotheubiquitinationsiteonhumanhistoneH2B.
        EpitopeLys120ubiquitinationsite
        CloneClone56
        HostMouse
        SpecificityRecognizeshistoneH2Bubiquitinatedonlysine120butnotfreeubiquitinorhistoneH2Binitsunmodifiedstate.
        IsotypeIgG2aκ
        SpeciesReactivity
        • Mouse
        • Human
        • Vertebrates
        • Rat
        • GreenPlants
        SpeciesReactivityNoteHuman,mouse,rat.Nottestedinotherspecies.Theimmunogensequenceisidenticalinawiderangeofanimalandplantspecies,sobroadcross-reactivityisexpected.
        AntibodyTypeMonoclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneencodesamemberofthehistoneH2Bfamily,andgeneratestwotranscriptsthroughtheuseoftheconservedstem-loopterminationmotif,andthepolyAadditionmotif.[providedbyRefSeq]
        GeneSymbol
        • H2B.1
        • H2B.a/g/h/k/l
        • H2B/a
        • H2B/g
        • H2B/h
        • H2B/k
        • H2B/l
        • H2BFA
        • H2BFG
        • H2BFH
        • H2BFK
        • H2BFL
        Modifications
        • Ubiquitylation
        UniProtNumber
        UniProtSummaryFUNCTION:Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.

        Hasbroadantibacterialactivity.Maycontributetotheformationofthefunctionalantimicrobialbarrierofthecolonicepithelium,andtothebactericidalactivityofamnioticfluid.

        SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.

        SUBCELLULARLOCATION:Nucleus.

        PTM:MonoubiquitinationofLys-120bytheRNF20/40complexgivesaspecifictagforepigenetictranscriptionalactivationandisalsoprerequisiteforhistoneH3"Lys-4"and"Lys-79"methylation.ItalsofunctionscooperativelywiththeFACTdimertostimulateelongationbyRNApolymeraseII.

        PhosphorylatedonSer-15bySTK4/MST1duringapoptosis;whichfacilitatesapoptoticchromatincondensation.AlsophosphorylatedonSer-15inresponsetoDNAdoublestrandbreaks(DSBs),andincorrelationwithsomatichypermutationandimmunoglobulinclass-switchrecombination.

        SEQUENCESIMILARITY:BelongstothehistoneH2Bfamily.

        MolecularWeight~25kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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