• <menu id="uuusw"></menu>
    •
  • <table id="uuusw"></table>
    當前位置 ： Millipore >>> Millipore/17-480 | Phospho-FAK (Tyr397) STAR ELISA Kit/17-480/96 assays
    Millipore/17-480 | Phospho-FAK (Tyr397) STAR ELISA Kit/17-480/96 assays
    • Millipore/17-480 | Phospho-FAK (Tyr397) STAR ELISA Kit/17-480/96 assays

    Millipore/17-480 | Phospho-FAK (Tyr397) STAR ELISA Kit/17-480/96 assays

    價格: 試用 市場價: 0.00
    打印產品信息
    貨號: 17-480
    品牌: Millipore
    規格
    數量
    庫存(0)
    特別 提示
    代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
    下單后請及時聯系客服核對商品價格，訂單生效后再付款。
    立即申請

    收藏商品
    資深產品顧問
    咨詢顧問

    全國免費服務熱線

    4000-520-616

    • 自營商城 一站式服務
    • 廠家直采 剔除溢價
    • 品質甄選 正品保證
    • 嚴控流程 只做188精品
    • 極速物流 如約送貨
    • 詳情
    • 使用說明
    • 常見問題
      • Description
        CatalogueNumber17-480
        BrandFamilyUpstate
        TradeName
        • STAR
        • Upstate
        DescriptionPhospho-FAK(Tyr397)STARELISAKit
        AlternateNames
        • Focaladhesionkinase1
        • FADK1
        • Focaladhesionkinase-relatednonkinase
        • FRNK
        • Proteinphosphatase1regulatorysubunit71
        • PPP1R71
        • Protein-tyrosinekinase2
        • p125FAK
        • pp125FAK
        BackgroundInformationFAK(FocalAdhesionKinase)isanon-receptortyrosinekinasewhichlocalizestofocaladhesionsandplaysakeyroleinintegrin-mediatedsignalingpathwaysregulatingcellmigration.Itautophosphorylatesontyrosine397duringadhesionandspreADIng,andisthoughttobeacriticaltransducerofadhesion-dependentgrowthandsurvival.FAKanditphosphorylationstateshavebeenimplicatedincancermetastasisandtumorcellsurvivalandadhesion-independentgrowth.Additionally,recentevidenceindicatesthatelevationofFAKactivityinhumancarcinomacellsisassociatedwithincreasedinvasivepotential.AcentralroleintumorformationandprogressionsuggestthatFAKisanattractivetargetfortherapeuticintervention.Cellsurfaceintegrinswhenengagedwiththeirligandsleadtotherecruitmentofanumberofintracellularproteinstospecializedsitesofthecytoplasmicfaceintofocaladhesions.Thesefocaladhesionplaquesarecomposedofmanyproteinsthatincludekinases,phosphatases,scaffoldingproteins,andG-proteins.Theactivationofintegrinsleadstothetyrosinephosphorylationofseveralcytoplasmicproteinscriticaltothebiochemicalprocess,includingFAK,Src,paxillin,tensin,andp130Cas.Thisclusteringandactivationofproteinsatthemembraneleadstotheactivationofmanydivergingsignalingpathwaysthatresultincytoskeletalre-organization.ThisstartswitheithertherecruitmentofSrcandFAKtotheplasmamembranefollowingintegrinorPI3Kinaseactivation.Thenon-receptorprotein-tyrosinekinases(PTKs)FAKandSrcarekeymembersofthefocaladhesioncomplex.ThesePTKsco-Localizeandassociatewiththetransmembraneintegrinsandvariousdownstreamtargetsofcertaingrowthfactorreceptors.TheoutcomeofSrcbindingtoFAKisthephosphorylationofseveraltyrosineresiduesinFAKanditsrelatedproteins,includingtheFAKautophosphorylationsiteTyr397.ThisautophosphorylationoccursimmediatelyafterintegrinclusteringandallowsfortheassociationofFAKwithvarioussignalingproteinssuchasPI3kinase,Grb2-Sos,p130Cas,andpaxillin.ThephosphorylationofFAKTyr397iscrucialformanyoftheestablishedBIOLOGicalrolesofFAK,includingcellmigration,cellcycleprogression,andpreventionofanoikis,aformofdetachment-inducedapoptosisandiscorrelatedwiththeinvasivenessoftumorsandisthoughttobeapivotalplayerbymodulatingtheturnoveroffocaladhesionstoregulatecellmigration.Phosphoinositide3-Kinase(PI3kinaseorPI3K)alsoplaysaroleincellmigrationviaitsbindingtotheautophosphorylationsite(Tyr397)ofFAKthroughoneorbothofitsSH2domainsinthep85subunitofPI3K.ThisisthesamesiteinFAKthatisboundbySrc.Intheory,thebindingofPI3kinasetoFAKmaybeinvolvedinactivitieslikecellproliferation,apoptosis,andmigrationthatarerelatedtothephosphorylationofTyr397.ThisissubstantiatedbytheobservationthatAKTisdownstreamofPI3kinaseandisamediatorinpreventingapoptosis.ThereforeitispossIBLethatFAK/PI3kinaseassociationmayhelpregulateapoptosis.
        MaterialsRequiredbutNotDelivered1.Multi-channelorrepeatingPipettes2.Plateshaker(optional)3.Pipettorsandtipscapableofaccuratelymeasuring1-1000μL4.GraduatedSEROlogicalpipettes5.96-wellmicrotiterPlateReaderwith450nmfilter6.Graphingsoftwareforplottingdataorgraphpaperformanualplottingofdata7.Microfugetubesforstandardandsampledilutions8.Mechanicalvortex9.1litercontainer10.Distilledordeionizedwater
        ProductInformation
        Components
        • 1.CapturePlatepre-coatedwithanti-FAKantibody:(PartNo.17-480A)Onepre-coated96-stripwellimmunoplatesealedinafoilpouch.
        • 2.Anti-phospho-FAK(Tyr397)detectionantibody:(PartNo.17-480B)Onebottle(6mL)ofanti-phospho-FAK(Tyr397)detectionantibodycontainingsodiumazide,readytouse.
        • 3.ELISADiluent:(PartNo.17-480C)Onebottle(25mL)ofELISADiluentcontainingsodiumazide,readytouse.
        • 4.25XELISAWashBuffer:(PartNo.17-480D)Onebottle(50mL)of25XELISAWashBuffer.
        • 5.Anti-RabbitIgGHRPconjugate:(PartNo.17-480E)Onevial(125μL)of100Xanti-rabbitHRPconjugatecontainingthimerosal.
        • 6.HRPDiluent:(PartNo.17-480F)Onebottle(25mL)ofHRPDiluentcontainingthimerosal.
        • 7.TMBSolution:(PartNo.17-480G)Onebottle(25mL)ofstABIlizedtetramethylbenzidine(TMB),readytouse.
        • 8.StopSolution:(PartNo.17-480H)Onebottle(25mL)ofstopsolution,readytouse.
        • 9.phospho-FAK(Tyr397)Standard:(PartNo.17-480I)Twovialsofphospho-FAK(Tyr397)standard,lyophilized.
        • 10.PlateCovers:Twoplatecovers.
        DetectionmethodChromogenic
        StorageandShippingInformation
        StorageConditions1yearat4°Cfromdateofshipment
        Applications
        ApplicationThisPhospho-FAK(Tyr397)STARELISAKitisusedtomeasure&quantifyFAKlevels.
        KeyApplications
        • EnzymeAssays
        BiologicalInformation
        SpeciesReactivity
        • Human
        • Mouse
        • Rat
        AnalytesAvailable
        • FAK
        EntrezGeneNumber
        EntrezGeneSummaryThisgeneencodesacytoplasmicproteintyrosinekinasewhichisfoundconcentratedinthefocaladhesionsthatformbetweencellsgrowinginthepresenceofextracellularmatrixconstituents.TheencodedproteinisamemberoftheFAKsubfamilyofproteintyrosinekinasesbutlackssignificantsequencesimilaritytokinasesfromothersubfamilies.Activationofthisgenemaybeanimportantearlystepincellgrowthandintracellularsignaltransductionpathwaystriggeredinresponsetocertainneuralpeptidesortocellinteractionswiththeextracellularmatrix.Atleastfourtranscriptvariantsencodingfourdifferentisoformshavebeenfoundforthisgene,butthefull-lengthnaturesofonlytwoofthemhavebeendetermined.
        GeneSymbol
        • PTK2
        • pp125FAK
        • FADK
        • FAK1
        • FAK
        Modifications
        • Phosphorylation
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:Q05397#Non-receptorprotein-tyrosinekinaseimplicatedinsignalingpathwaysinvolvedincellmotility,proliferationandapoptosis.Activatedbytyrosine-phosphorylationinresponsetoeitherintegrinclusteringinducedbycelladhesionorantibodycross-linking,orviaG-proteincoupledreceptor(GPCR)occupancybyligandssuchasbombesinorlysophosphatidicacid,orviaLDLreceptoroccupancy.Playsapotentialroleinoncogenictransformationsresultinginincreasedkinaseactivity.
        SIZE:1052aminoacids;119233Da
        SUBUNIT:InteractswithCASfamilymembersandwithGIT1,SORBS1andBCAR3.InteractswithSHB(Bysimilarity).InteractswithTGFB1I1.
        SUBCELLULARLOCATION:Celljunction,focaladhesion;Cytoplasmicside.Note=Constituentoffocaladhesions.
        TISSUESPECIFICITY:Expressedinallorganstested,inlymphoidcelllines,butmostabundantlyinbrain.
        DOMAIN:SwissProt:Q05397ThefirstPro-richdomaininteractswiththeSH3domainofCRK-associatedsubstrate(BCAR1)andCASL(Bysimilarity).&Thecarboxy-terminalregionisthesiteoffocaladhesiontargeting(FAT)sequencewhichmediatesthelocalizationofFAK1tofocaladhesions.
        PTM:Phosphorylatedon6tyrosineresiduesuponactivation.
        SIMILARITY:Belongstotheproteinkinasesuperfamily.Tyrproteinkinasefamily.FAKsubfamily.&Contains1FERMdomain.&Contains1proteinkinasedomain.
        PhysicochemicalInformation
        Sensitivity
        • Sensitivity:1.6Units/mL.
          RangeofDetection:1.6to100Units/mL
        Dimensions
        MaterialsInformation
        MaterialsInformation
      售后保障
      螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。
    • Q：生物188產品正品保障嗎？
      A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購， 自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

      Q：下單后可以修改訂單嗎？
      A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

      Q：可以開發票嗎？
      A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

      Q：商品幾天可以發貨？
      A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

      Q：如何聯系商家？
      A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

      Q：收到的商品少了/發錯了怎么辦？
      A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是 部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

      Q：退換貨/維修需要多長時間？
      A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、 數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

    客戶服務

    在線客服
    用戶反饋

    何為188

    極簡而嚴謹，我們僅銷售188款生物醫學科研用品，款款都是爆款；因為少所以聚焦，聚焦甄選每一款產品，聚焦服務每一位客戶!

    關注我們 :

    點擊QQ聯系我們
    生物188微信

    關注188微信公眾號

    獲取最新優惠活動通知

    • 品質甄選，正品保證

    • 自營電商，廠家直采

    • 極簡主義，188精品

    常見問題 | 購買相關 | 配送與驗收 | 支付問題 | 售后與服務 | 服務協議 | 隱私政策 | 何為188 | 中國智造 | 聯系我們
    友情鏈接： ImmunoChem中國 | Paragon Genomics價格 | undergroundshop產品代理 | Matreya官網 | ezbiosystems代理 | 螞蟻淘 | 線粒體蛋白

    公司版權所有 ? 2005-2021 蘇州螞蟻淘生物科技有限公司 蘇ICP備17049038號-10

  • <menu id="uuusw"></menu>
    •
  • <table id="uuusw"></table>
    上床视频