• <menu id="uuusw"></menu>
    •
  • <table id="uuusw"></table>
    當前位置 ： Millipore >>> Millipore/17-10099 | ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set/17-10099/25 assays25 assa
    Millipore/17-10099 | ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set/17-10099/25 assays25 assa
    • Millipore/17-10099 | ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set/17-10099/25 assays25 assa

    Millipore/17-10099 | ChIPAb+ Acetyl-Histone H4 (Lys8) - ChIP Validated Antibody and Primer Set/17-10099/25 assays25 assa

    價格: ￥4800.00 市場價: 8000.00
    打印產品信息
    貨號: 17-10099
    品牌: Millipore
    規格
    數量
    庫存(0)
    特別 提示
    代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
    下單后請及時聯系客服核對商品價格，訂單生效后再付款。
    立即購買 加入購物車

    收藏商品
    資深產品顧問
    咨詢顧問

    全國免費服務熱線

    4000-520-616

    • 自營商城 一站式服務
    • 廠家直采 剔除溢價
    • 品質甄選 正品保證
    • 嚴控流程 只做188精品
    • 極速物流 如約送貨
    • 詳情
    • 使用說明
    • 常見問題
      • Description
        CatalogueNumber17-10099
        BrandFamilyUpstate
        TradeName
        • ChIPAb+
        • Upstate
        DescriptionChIPAb+Acetyl-HistoneH4(Lys8)-ChIPValidatedAntibodyandPrimerSet
        OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
        TheChIPAb+Acetyl-HistoneH4(Lys8)setincludestheAcetyl-HistoneH4(Lys8)antibody,anegativecontrolrabbitserum,andqPCRprimerswhichamplifya134bpregionofhumanHSPCA.TheAcetyl-HistoneH4(Lys8)andnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofAcetyl-HistoneH4(Lys8)-associatedchromatin.
        AlternateNames
        • H4K8Ac
        • HistoneH4(acetylK8)
        BackgroundInformationHistoneH4isoneofthe5mainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongNterminaltailH4isinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.

        TheN-terminaltailofhistoneH4protrudesfromtheglobularnucleosomecoreandcanundergoseveraldifferenttypesofepigeneticmodificationsthatinfluencecellularprocesses.Thesemodificationsincludethecovalentattachmentofmethyloracetylgroupstolysineandarginineaminoacidsandthephosphorylationofserineorthreonine.
        ProductInformation
        FormatSerum
        Control
        • IncludesnegativecontrolnormalrabbitserumantibodyandprimersspecificforhumanHSPCA.
        PresentationAnti-Acetyl-HistoneH4(Lys8)(rabbitpolyclonal).Onevialcontaining50μLofantiserumcontaining0.05%sodiumazide.Storeat-20°C.

        NormalRabbitSerum.Onevialcontaining100μLofantiserumcontaining0.05%sodiumazide.
        Storeat-20°C.

        ChIPPrimers,HSPCA.Onevialcontaining75μLof5μMofeachprimerspecificforhumanHSPCA.
        Storeat-20°C.
        FOR:GCAACAGCTACCACAGGACCA
        REV:GAGCGTGTGAAATCAACATAAAGC
        StorageandShippingInformation
        StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
        Applications
        ApplicationTheChIPAb+Acetyl-HistoneH4(Lys8)setincludestheAcetyl-HistoneH4(Lys8)antibody,anegativecontrolrabbitserum&qPCRprimerswhichamplifya134bpregionofhumanHSPCA.
        KeyApplications
        • WesternBlotting
        • ChromatinImmunoprecipitation(ChIP)
        ApplicationNotesChromatinImmunoprecipitation:
        SonicatedchromatinpreparedfromHeLacells(1X106cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusingeither2μLofNormalRabbitSerumor2μLofAnti-Acetyl-HistoneH4(Lys8)andtheMagnaChIP?AKit(Cat.#17-610).
        SuccessfulimmunoprecipitationofAcetyl-HistoneH4(Lys8)associatedDNAfragmentswasverifiedbyqPCRusingChIPPrimers,humanHSPCAforapositive,andknownNegativePrimers(Martinato,F.,etal.,2008)asanegativeassay(Pleaseseefigures).
        DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.
        PleaserefertotheEZ-MagnaChIP?A(Cat.#17-408)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails.

        WesternBlotAnalysis:
        Representativelotdata.
        SodiumButyrate-treatedHeLaacidextractwasresolvedbyelectrophoresis,transferredtoPVDFmembraneandprobedwithAnti-AcetylHistoneH4(Lys8)(1:1000dilution).Todemonstratespecificity,serumwaspreincubatedwithmodifiedHistoneH4peptides:
        Lane1:Nopeptide
        Lane2:acetyl-HistoneH4(K5)(Cat.#:12-343)
        Lane3:acetyl-HistoneH4(K8)(Cat.#:12-344)
        Lane4:acetyl-HistoneH4(K12)(Cat.#:12-345)
        Lane5:acetyl-HistoneH4(K16)(Cat.#:12-346)
        Lane6:acetyl-HistoneH4(K5,8,12,16)(Cat.#:12-353)
        Lane7:HistoneH4(Cat.#:12-347)
        Proteinswerevisualizedusingadonkeyanti-rabbitIgGconjugatedtoHRPandvisualizedusingachemiluminescencedetectionsystem(Pleaseseefigures).

        Immunoprecipitation:5μLofapreviouslotimmunoprecipitatedacetylHistoneH4from1mgofwildtypewholecellyeastlysatesbutnotfromayeaststraincontainingaHistoneH4Lys8Rsubstitution.
        BiologicalInformation
        ImmunogenSyntheticpeptidecorrespondingtoyeastHistoneH4acetylatedatlysine8.
        HostRabbit
        SpecificityRecognizesacetyl-HistoneH4(Lys8).
        SpeciesReactivity
        • Human
        • Yeast(S.cerevisiae)
        SpeciesReactivityNoteDemonstratedtoreactwithYeastandhuman.Broadspeciescross-reactivityisexpectedbasedon100%sequencehomology.
        AntibodyTypePolyclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Twomoleculesofeachofthefourcorehistones(H2A,H2B,H3,andH4)formanoctamer,aroundwhichapproximately146bpofDNAiswrappedinrepeatingunits,callednucleosomes.Thelinkerhistone,H1,interactswithlinkerDNAbetweennucleosomesandfunctionsinthecompactionofchromatinintohigherorderstructures.ThisgeneisintronlessandencodesamemberofthehistoneH4family.TranscriptsfromthisgenelackpolyAtailsbutinsteadcontainapalindromicterminationelement.Thisgeneisfoundinthelargehistonegeneclusteronchromosome6.
        GeneSymbol
        • HIST1H4A
        • H4/J
        • HIST1H4F
        • HIST1H4L
        • H4FN
        • H4FA
        • H4FH
        • HIST1H4H
        • H4F2
        • H4FM
        • H4/A
        • H4FK
        • H4FG
        • HIST2H4
        • H4FB
        • H4/K
        • HIST1H4I
        • H4/N
        • HIST1H4B
        • H4FE
        • H4/M
        • H4/a
        • HIST1H4E
        • HIST4H4
        • HIST2H4A
        • H4FC
        • H4FI
        • H4/H
        • HIST1H4C
        • H4/G
        • HIST1H4K
        • H4FJ
        • H4FD
        • H4/I
        • H4/B
        • H4/D
        • H4/C
        • HIST1H4J
        • HIST1H4D
        • H4/E
        Modifications
        • Acetylation
        PurificationMethodUnpurified
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:P62805#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstABIlity.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.
        SIZE:103aminoacids;11367Da
        SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.
        SUBCELLULARLOCATION:Nucleus.
        PTM:SymmetricdimethylationonArg-4bythePRDM1/PRMT5complexmayplayacrucialroleinthegerm-celllineage(Bysimilarity).&UbiquitinatedbytheCUL4-DDB-RBX1complexinresponsetoultravioletirrADIation.ThismayweakentheinteractionbetweenhistonesandDNAandfacilitateDNAaccessibilitytorepairproteins.
        SIMILARITY:SwissProt:P62805##BelongstothehistoneH4family.
        MolecularWeight~11kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
      售后保障
      螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。
    • Q：生物188產品正品保障嗎？
      A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購， 自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

      Q：下單后可以修改訂單嗎？
      A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

      Q：可以開發票嗎？
      A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

      Q：商品幾天可以發貨？
      A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

      Q：如何聯系商家？
      A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

      Q：收到的商品少了/發錯了怎么辦？
      A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是 部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

      Q：退換貨/維修需要多長時間？
      A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、 數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

    客戶服務

    在線客服
    用戶反饋

    何為188

    極簡而嚴謹，我們僅銷售188款生物醫學科研用品，款款都是爆款；因為少所以聚焦，聚焦甄選每一款產品，聚焦服務每一位客戶!

    關注我們 :

    點擊QQ聯系我們
    生物188微信

    關注188微信公眾號

    獲取最新優惠活動通知

    • 品質甄選，正品保證

    • 自營電商，廠家直采

    • 極簡主義，188精品

    常見問題 | 購買相關 | 配送與驗收 | 支付問題 | 售后與服務 | 服務協議 | 隱私政策 | 何為188 | 中國智造 | 聯系我們
    友情鏈接： Immunomedics中國 | Parkell價格 | agrochemicals華東代理 | Maxim Biomedical廠家 | ezbiosystems代理 | 螞蟻淘 | 細胞分析系統

    公司版權所有 ? 2005-2021 蘇州螞蟻淘生物科技有限公司 蘇ICP備17049038號-10

  • <menu id="uuusw"></menu>
    •
  • <table id="uuusw"></table>
    上床视频