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    當前位置 ： Millipore >>> Millipore/17-10054 | ChIPAb+ Histone H2B - ChIP Validated Antibody and Primer Set/17-10054/25 assays25 assays per set. R
    Millipore/17-10054 | ChIPAb+ Histone H2B - ChIP Validated Antibody and Primer Set/17-10054/25 assays25 assays per set. R
    • Millipore/17-10054 | ChIPAb+ Histone H2B - ChIP Validated Antibody and Primer Set/17-10054/25 assays25 assays per set. R

    Millipore/17-10054 | ChIPAb+ Histone H2B - ChIP Validated Antibody and Primer Set/17-10054/25 assays25 assays per set. R

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    貨號: 17-10054
    品牌: Millipore
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      • Description
        CatalogueNumber17-10054
        BrandFamilyUpstate
        TradeName
        • ChIPAb+
        • Upstate
        DescriptionChIPAb+HistoneH2B-ChIPValidatedAntibodyandPrimerSet
        OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
        TheChIPAb+HistoneH2BsetincludestheHistoneH2Bantibody,anegativecontrolrabbitIgG,andqPCRprimerswhichamplifya110bpregionofhumanβ-globinpromoter.TheHistoneH2Bandnegativecontrolsaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHistoneH2B-associatedchromatin.
        AlternateNames
        • H2B
        • HistoneH2B
        • H2Bhistonefamily,memberQ
        • histone2,H2be
        • histonecluster2,H2be
        BackgroundInformationHistoneH2Bisoneofthe5mainhistoneproteinsinvolvedinthestructureofchromatinineukaryoticcells.FeaturingamainglobulardomainandalongNterminaltailH2Aisinvolvedwiththestructureofthenucleosomesofthe"beadsonastring"structure.
        ProductInformation
        FormatPurified
        Control
        • IncludesnegativecontrolnormalrabbitIgGantibodyandprimersspecificforhumanβ-globin.
        PresentationAnti-HistoneH2B(rabbitpolyclonal).Onevialcontaining25μLofproteinApurifiedrabbitpolyclonalinbuffercontaining0.02Mphosphatebuffer,pH7.6,0.25MNaCl,and0.1%sodiumazide,and30%glycerol.Storeat-20°C.

        NormalRabbitIgG.Onevialcontaining125μgofpurifiedrabbitIgGin125μLstoragebuffer.Storeat-20°C.

        ChIPPrimers,β-globin.Onevialcontaining75μLof5μMofeachprimerspecificforthehumanβ-globinpromoter.Storeat-20°C.
        FOR:AGGACAGGTACGGCTGTCATC
        REV:TTTATGCCCAGCCCTGGCTC
        StorageandShippingInformation
        StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:VariABIllityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
        Applications
        ApplicationTheChIPAb+HistoneH2BsetincludestheHistoneH2Bantibody,anegativecontrolrabbitIgG&qPCRprimerswhichamplifya110bpregionofhumanβ-globinpromoter.
        KeyApplications
        • WesternBlotting
        • ChromatinImmunoprecipitation(ChIP)
        ApplicationNotesChromatinImmunoprecipitation:
        SonicatedchromatinpreparedfromHeLacells(1X106cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusingeither1μLofNormalRabbitIgGor1μLofAnti-HistoneH2BandtheMagnaChIP?AKit(Cat.#17-610).
        SuccessfulimmunoprecipitationofHistoneH2BassociatedDNAfragmentswasverifiedbyqPCRusingChIPPrimers,humanβ-globinforapositive,andhumanGAPDHprimersasanegativeassay(Pleaseseefigures).
        DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.
        PleaserefertotheEZ-MagnaChIP?A(Cat.#17-408)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails.

        WesternBlotAnalysis:
        Representativelotdata.
        RecombinantXenopusHistoneH2B,chickencorehistonesandacidextractfromeithersodiumbutyrateorcolcemidtreatedHeLacellswereresolvedbyelectrophoresis,transferredtonitrocelluloseandprobedwithanti-HistoneH2B(1:5,000dilution).
        Proteinswerevisualizedusingagoatanti-rabbitsecondaryantibodyconjugatedtoHRPandachemiluminescencedetectionsystem(Pleaseseefigures).
        BiologicalInformation
        ImmunogenKLH-conjugated,syntheticpeptidecorrespondingtoaminoacids118-126(CG-AVTKYTSSK)ofhumanHistoneH2B,withanN-terminalCGaddedforconjugationpurposes.
        HostRabbit
        SpecificityRecognizesandisspecificforHistoneH2B,MW~15kDa.Anadditionalbandat~23kDaisdetectedinsomesamples,whichlikelycorrespondstoubiquityl-HistoneH2B.
        SpeciesReactivity
        • Chicken
        • Xenopus
        • Human
        • Yeast(S.cerevisiae)
        SpeciesReactivityNoteHuman,chicken,yeastandXenopus.Othersnottested.Broadspeciescross-reactivityexpectedbasedonsequencehomology.
        AntibodyTypePolyclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneisintronlessandencodesamemberofthehistoneH2Bfamily.Transcriptsfromthisgenecontainapalindromicterminationelement.
        GeneSymbol
        • GL105
        • H2B
        • H2B-GL105
        • H2B.1
        • H2B.q
        • H2B/q
        • H2BFQ
        • MGC119802
        • MGC119804
        • MGC129733
        • MGC129734
        • OTTHUMP00000013920
        PurificationMethodProteinAPurfied
        UniProtNumber
        UniProtSummaryFUNCTION:SwissProt:Q8N257#Corecomponentofnucleosome.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.
        SIZE:126aminoacids;13908Da
        SUBUNIT:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.
        SUBCELLULARLOCATION:Nucleus.
        PTM:MonoubiquitinationofLys-121bytheRNF20/40complexgivesaspecifictagforepigenetictranscriptionalactivationandisalsoprerequisiteforhistoneH3"Lys-4"and"Lys-79"methylation.ItalsofunctionscooperativelywiththeFACTdimertostimulateelongationbyRNApolymeraseII.&PhosphorylatedonSer-15bySTK4/MST1duringapoptosis;whichfacilitatesapoptoticchromatincondensation.AlsophosphorylatedonSer-15inresponsetoDNAdoublestrandbreaks(DSBs),andincorrelationwithsomatichypermutationandimmunoglobulinclass-switchrecombination.
        SIMILARITY:SwissProt:Q8N257##BelongstothehistoneH2Bfamily.
        MolecularWeight~15kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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