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    當前位置 ： Millipore >>> Millipore/17-10048 | ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set/17-10048/25 assays25 assays per set.
    Millipore/17-10048 | ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set/17-10048/25 assays25 assays per set.
    • Millipore/17-10048 | ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set/17-10048/25 assays25 assays per set.

    Millipore/17-10048 | ChIPAb+ Histone H2A.Z - ChIP Validated Antibody and Primer Set/17-10048/25 assays25 assays per set.

    價格: ￥4800.00 市場價: 8000.00
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    貨號: 17-10048
    品牌: Millipore
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      • Description
        CatalogueNumber17-10048
        BrandFamilyUpstate
        TradeName
        • ChIPAb+
        • Upstate
        DescriptionChIPAb+HistoneH2A.Z-ChIPValidatedAntibodyandPrimerSet
        OverviewAllChIPAb+antibodiesareindividuallyvalidatedforchromatinprecipitation,everylot,everytime.EachChIPAb+antibodysetincludescontrolprimers(testedeverylotbyqPCR)toBIOLOGicallyvalidateyourIPresultsinalocus-specificcontext.TheqPCRprotocolandprimersequencesareprovided,allowingresearcherstovalidateChIPprotocolswhenusingourantibodyintheirchromatincontext.EachsetalsoincludesanegativecontrolantibodytoensurespecificityoftheChIPreaction.
        TheChIPAb+HistoneH2A.ZsetincludestheHistoneH2A.Zantibody,anegativecontrolantibody(normalsheepIgG),andqPCRprimerswhichamplifya166bpregionofhumanGAPDH.TheHistoneH2A.Zandnegativecontrolantibodiesaresuppliedinascalable"perChIP"reactionsizeandcanbeusedtofunctionallyvalidatetheprecipitationofHistoneH2A.Z-associatedchromatin.
        AlternateNames
        • H2Ahistonefamily,memberZ
        • H2AZhistone
        BackgroundInformationH2A.Z,alsoknownasH2AFZ,isavariantofhistoneH2Avariantthatishighlyconservedfromyeasttohumans.Likeotherhistonevariants,H2A.Zisencodedbyauniquegene,resultinginaslightlydivergenttypeofH2Awithspecializedfunctions.H2A.ZisinvolvedinchromatincompactionbystABIlizingthehistoneoctamerwithinthenucleosome.Itpromotesgeneexpressionthroughthecreationofanopendomainofchromatinthatismoreeasilytranscribed.H2A.Zisnonrandomlydistributedthroughoutthegenome,targetedprimarilytopericentricheterochromatin,butexcludedfromtheinactiveXchromosomeandthenucleolus.H2A.Zisassociatedwithbotheuchromatinandfacultativeheterochromatin.DNAmethylationcaninfluencechromatinstructureandeffectgenesilencingbyexcludingH2A.Z,andlikewise,H2A.ZprotectsgenesfromDNAmethylation.Thus,H2A.Zplaysaroleinregulatingheterochromatinsilencingandisinvolvedintranscriptionalcontrol.
        ProductInformation
        FormatAffinityPurified
        Control
        • IncludesnegativecontrolsheepIgGantibodyandprimersspecificforhumanGAPDH.
        PresentationAnti-HistoneH2A.Z(SheeppolyclonalIgG).Onevialcontaining25μgofaffinitypurifiedsheeppolyclonalIgGinPBS,pH7.4containing1%BSAwith0.02%sodiumazide,beforetheadditionof30%glycerol.Storeat-20°C.

        NormalSheepIgG.Onevialcontaining25μgofproteinGpurifiedsheepIgGin0.02Mpotassiumphosphate,0.15MNaCl,pH7.2,with0.01%sodiumazide,beforetheadditionof30%glycerol.Storeat-20°C.

        ControlPrimers.Onevialcontaining75μLof5μMofeachprimerspecificforhumanGAPDH.Storeat-20°C.
        FOR:TACTAGCGGTTTTACGGGCG
        REV:TCGAACAGGAGGAGCAGAGAGCGA
        StorageandShippingInformation
        StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.HandlingRecommendations:Uponfirstthaw,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.Note:Variabilityinfreezertemperaturesbelow-20°Cmaycauseglycerolcontainingsolutionstobecomefrozenduringstorage.
        Applications
        ApplicationThisChIPAb+HistoneH2A.Z-ChIPValidatedAntibody&PrimerSetconvenientlyincludestheantibody&thespecificcontrolPCRprimers.
        KeyApplications
        • ChromatinImmunoprecipitation(ChIP)
        • WesternBlotting
        ApplicationNotesChromatinImmunoprecipitation:
        SonicatedchromatinpreparedfromHeLacells(1X106cellequivalentsperIP)weresubjectedtochromatinimmunoprecipitationusing1μgofeitherNormalSheepIgGor1μgofAnti-HistoneH2A.ZandtheMagnaChIP?GKit(Cat.#17-611).
        SuccessfulimmunoprecipitationofHistoneH2A.ZassociatedDNAfragmentswasverifiedbyqPCRusingControlPrimers(PartNo.22-004)forapositive,andGAPDHCodingprimersasanegativeassay(Pleaseseefigures).
        DataispresentedaspercentinputofeachIPsamplerelativetoinputchromatinforeachampliconandChIPsampleasindicated.
        PleaserefertotheEZ-MagnaChIP?A(Cat.#17-408)orEZ-ChIP?(Cat.#17-371)protocolforexperimentaldetails.

        WesternBlotAnalysis:
        Representativelotdata
        HeLalysatewasresolvedbyelectrophoresis,transferredtoPVDFandprobedwithanti-HistoneH2A.Z(1:1000dilution).
        Proteinswerevisualizedusingarabbitanti-sheepsecondaryantibodyconjugatedtoHRPandachemiluminescencedetectionsystem(Pleaseseefigures).
        BiologicalInformation
        ImmunogenLinearpeptidecorrespondingtheC-terminalaminoacids113-127ofhumanHistoneH2A.Z.
        EpitopeC-terminus
        HostSheep
        SpecificityRecognizesHistoneH2A.Z,Mr~15kDa.
        SpeciesReactivity
        • Human
        SpeciesReactivityNoteHuman.Widespeciesreactivityisexpected.
        Predictedtocross-reactwithzebrafish,mouse,chicken,rat,rabbit,andXenopusbasedonsequencehomology.
        AntibodyTypePolyclonalAntibody
        EntrezGeneNumber
        EntrezGeneSummaryHistonesarebasicnuclearproteinsthatareresponsIBLeforthenucleosomestructureofthechromosomalfiberineukaryotes.Nucleosomesconsistofapproximately146bpofDNAwrappedaroundahistoneoctamercomposedofpairsofeachofthefourcorehistones(H2A,H2B,H3,andH4).Thechromatinfiberisfurthercompactedthroughtheinteractionofalinkerhistone,H1,withtheDNAbetweenthenucleosomestoformhigherorderchromatinstructures.ThisgeneencodesamemberofthehistoneH2Afamily.Severaltranscriptvariantsencodingdifferentisoforms,havebeenidentifiedforthisgene.[providedbyRefSeq].
        GeneSymbol
        • H2A.z
        • H2A/z
        • H2AZ
        • MGC117173
        • H2AFZ
        PurificationMethodAffinityPurfied
        UniProtNumber
        UniProtSummaryFUNCTION:VarianthistoneH2AwhichreplacesconventionalH2Ainasubsetofnucleosomes.NucleosomeswrapandcompactDNAintochromatin,limitingDNAaccessibilitytothecellularmachinerieswhichrequireDNAasatemplate.Histonestherebyplayacentralroleintranscriptionregulation,DNArepair,DNAreplicationandchromosomalstability.DNAaccessibilityisregulatedviaacomplexsetofpost-translationalmodificationsofhistones,alsocalledhistonecode,andnucleosomeremodeling.Maybeinvolvedintheformationofconstitutiveheterochromatin.Mayberequiredforchromosomesegregationduringcelldivision.Ref.7

        SUBUNITSTRUCTURE:ThenucleosomeisahistoneoctamercontainingtwomoleculeseachofH2A,H2B,H3andH4assembledinoneH3-H4heterotetramerandtwoH2A-H2Bheterodimers.Theoctamerwrapsapproximately147bpofDNA.H2AoritsvariantH2AFZformsanheterodimerwithH2B.H2AFZinteractswithINCENPBysimilarity.

        SUBCELLULARLOCATION:Nucleus.

        PTM:MonoubiquitinationofLys-122givesaspecifictagforepigenetictranscriptionalrepression.

        AcetylatedonLys-5,Lys-8andLys-12duringinterphase.AcetylationdisappearsatmitosisBysimilarity.

        NotphosphorylatedBysimilarity.

        SIMILARITY:BelongstothehistoneH2Afamily.

        MOLECULARWEIGHT:is13413.4Dafrompositions2-128.DeterminedbyESI.Monoisotopic,notmodified.Ref.8Ref.10Ref.11

        MolecularWeight~15kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
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