• <menu id="uuusw"></menu>
    •
  • <table id="uuusw"></table>
    當前位置 ： Millipore >>> Millipore/15-107 | MAPK Pathway 1 Explorer Antibody MiniPack/15-107/3 vials/Pk
    Millipore/15-107 | MAPK Pathway 1 Explorer Antibody MiniPack/15-107/3 vials/Pk
    • Millipore/15-107 | MAPK Pathway 1 Explorer Antibody MiniPack/15-107/3 vials/Pk

    Millipore/15-107 | MAPK Pathway 1 Explorer Antibody MiniPack/15-107/3 vials/Pk

    價格: ￥2832.00 市場價: 4720.00
    打印產品信息
    貨號: 15-107
    品牌: Millipore
    規格
    數量
    庫存(0)
    特別 提示
    代購產品：無質量問題不接受退換貨，下單前請仔細核對信息。
    下單后請及時聯系客服核對商品價格，訂單生效后再付款。
    立即購買 加入購物車

    收藏商品
    資深產品顧問
    咨詢顧問

    全國免費服務熱線

    4000-520-616

    • 自營商城 一站式服務
    • 廠家直采 剔除溢價
    • 品質甄選 正品保證
    • 嚴控流程 只做188精品
    • 極速物流 如約送貨
    • 詳情
    • 使用說明
    • 常見問題
      • Description
        CatalogueNumber15-107
        BrandFamilyUpstate
        TradeName
        • Upstate
        DescriptionMAPKPathway1ExplorerAntibodyMiniPack
        AlternateNames
        • OncogeneRAF1
        • rafproto-oncogeneserine/threonineproteinkinase
        BackgroundInformationPathwayExplorerAntibodyMiniPack:
        EachPathwayExplorerAntibodyMinipackcontainsthreerelatedantibodiesaspartofasignalingcascadeoracombinationoftotalandphosphorylatedformsofkeysignalingtargets.Eachofthethreeantibodiesare30%theoriginalpacksize.FullsizeversionsofeachofthePathwayExplorerantibodiesareavailableforsaleindividuallyunderthesamecatalognumberwiththeremovalof“SP”offofeachone(e.g.05-591SPcanbeorderedas05-591).

        Erk1/2:
        Erk(Extracellularsignal-RelatedKinase)isafamilyoftwo,highlyhomologousproteinsdenotedasErk1(p44,MAPK3)andErk2(p42,MAPK1)thatbothfunctioninthesamepathway.ThetwoproteinsareoftenreferredtocollectivelyasErk1/2orp44/p42MAPkinase.TheErkpathwayisconsideredtheclassical,canonicalMAPK(Mitogen-ActivatedProteinKinase)signalingpathway.Itisanevolutionarilyconservedpathwaythatcontrolsandisacriticalregulatorofthegrowthandsurvivalthroughthepromotionofcellproliferationandthepreventionofapoptosis.Erkisinvolvedinthecontrolofmanyfundamentalcellularprocessesincludingcellproliferation,survival,differentiation,apoptosis,motilityandmetabolism.Erkisactivatedbygrowthfactorstimulationofreceptortyrosinekinases(RTKs),GPCR,and/orintegrinstimulation.ThisactivatestheRas-Raf-MEK-Erkpathwaythatresultsinthephosphorylation/activationofErk1/2(p44/p42)ontheTxYmotif(Thr202/Tyr204andThr185/Tyr187forErk1&Erk2,respectively).

        Raf:
        TheRafproteins(A-Raf,B-Raf,Raf-1/c-Raf)areSer/ThrkinaseswithhomologytothePKCfamily,containinganN-terminalregulatorydomainandaC-terminalcatalyticdomain.Raf(MAPKinaseKinaseKinase)isthetopkinaseinthecanonicalMAPKpathway.MembersoftheRaffamilybindtoactivatedRas.Ras,initsGTP-boundactivestate,activatesthekinaseactivityofRaf.ThisresultsinRaftranslocationtotheplasmamembraneandactivation.Onceactivated,RafthenbindstoandactivatesMEK(MAPKinaseKinase)byphosphorylatingitonthetworesiduemotif.ItisthoughtthatB-RafmightbethepredominantactivatorofMEKandthatRaf-1hasaroleinprotectionagainstapoptosis;aprocessthatdoesnotrequireeitheritskinaseactivityoritsactivationofMEK.TheregulationofalargenumberofcellularprocessesaredependentupontheactivationstateofERK,sothecontrollingofthispathwaycouldhaveprofoundeffectsonvariousdiseases.ActivationofRaf-1involvesphosphorylationofSer338/339andTyr340/341.ActivatingmutationsofB-Rafthatdisruptitsauto-inhibitionloophavebeenimplicatedinanumberofcancers,includingmelanomaandcoloncancer.

        MEK1/MEK2:
        MAPKinase/ErkKinase(MEK),alternativelyknownasMKK,isatruedual-specificitykinase,inthatitphosphorylatestheMAPkinases(Erk1/2;p44/p42))onboththeThrandTyroftheactivationmotifTEY.Invitro,thetyrosinephosphorylationisfavored,whereasinvivobothphosphorylationeventsappeartooccursimultaneously.Thissuggeststhatanadditionalfactorispresentincellstofacilitatethereaction.MEK1andMEK2areactivatedbyphosphorylationoftwoserineresidues(Ser218/222inMEK1andSer222/226inMEK2),whicharesubstratesfortheRaffamilyofkinases.MutationofthephosphorylationsitesfromSertoAspcreatesaproteinwithconstitutivekinaseactivity,whichwhenexpressedincellsisabletocausetransformation.


        *Seefullsizeversionsforcorrespondingreferences.
        ProductInformation
        Components
        • 05-538SPAnti-phospho-Raf-1(Ser338)
        • 05-747SPAnti-phospho-MEK1(Ser218/222)/MEK2(Ser222/226)
        • 05-797RSPAnti-phospho-Erk1/2
          (Thr202/Tyr204,Thr185/Tyr187),recombinantcloneAW39R
        FormatPurified
        Control
        • 05-538SP:3T3/NIHcells
        Presentation3individualtubescontainingeitherAnti-phospho-Raf-1(Ser338);orAnti-phospho-MEK1(Ser218/222)/MEK2(Ser222/226);Anti-phospho-Erk1/2
        (Thr202/Tyr204,Thr185/Tyr187),recombinantcloneAW39R
        PropertiesEachvialis30%thesizeoftheparentcatalognumber
        StorageandShippingInformation
        StorageConditionsStablefor1yearat-20°Cfromdateofreceipt.
        HandlingRecommendations:Uponreceipt,andpriortoremovingthecap,centrifugethevialandgentlymixthesolution.Aliquotintomicrocentrifugetubesandstoreat-20°C.Avoidrepeatedfreeze/thawcycles,whichmaydamageIgGandaffectproductperformance.
        Applications
        ApplicationThisAntibodypackcontainsAnti-phospho-Raf-1Antibody(Ser338),Anti-phospho-MEK1Antibody(Ser218/222)/MEK2(Ser222/226),Anti-phospho-Erk1/2Antibody(Thr202/Tyr204,Thr185/Tyr187),validatedforuseinIF,Flow,WB,Multiplexing
        KeyApplications
        • Immunofluorescence
        • FlowCytometry
        • WesternBlotting
        • Multiplexing
        ApplicationNotes05-797RSP:ImmunofluorescenceAnalysis:A431cellswerefixed,permABIlizedanddualstainedwithanti-phospho-Erk.

        FlowCytometryAnalysis:Jurkatcellswerefixed,permeablized,andstainedwithanti-phospho-Erk1/2(Thr202/Tyr204,Thr185/Tyr187).


        BIOLOGicalInformation
        Immunogen05-797RSP:PhosphorylatedpeptideencompassingtheTxYmotifintheactivationloopofErk1andErk2.;05-747SP:KLH-conjugated,syntheticpeptidecontaining…DpSMANpSF…inwhichpScorrespondstophosphorylatedaminoacids218and222ofhumanMEK1.Theimmunizingsequenceishighlyconserved.;05-538SP:KLH-conjugated,syntheticpeptideencompassingandincludingphosphorylatedSer338ofhumanRaf-1.Theimmunizingsequenceisidenticalinratandchickenandhas12/13identicalaminoacidsinXenopus.
        EpitopePleaseseerelevantdatasheet.
        Clone05-797RSP:AW39R
        Concentration05-538SP:1mg/mL
        Specificity05-797RSP:RecognizesErk1&2onlywhenduallyphosphorylatedonitsTxYactivationmotif.;05-747SP:PhosphorylatedMEK1/MEK2;05-538SP:RecognizesRaf-1phosphorylatedatSerine338
        SpeciesReactivity
        • Human
        • Mouse
        • Rat
        • Chicken
        • Xenopus
        SpeciesReactivityNote05-538SP:Mouseandhuman.
        Predictedtocrossreactwithrat,chickenandXenopusbasedonsequencehomology.
        EntrezGeneNumber
        GeneSymbol
        • MAP2K1
        • PRKMK1
        • MKK1
        • MAPKK1
        • MEK1
        • RAF1
        • cRaf
        • Raf-1
        • c-Raf
        • RAF
        • CRAF
        • C-RAF
        • MAPK3
        • ERK1
        • p44
        • P44ERK
        • P44MAPK
        • Erk2
        • MAPK1
        • P42MAPK
        • P42ERK
        Modifications
        • Phosphorylation
        UniProtNumber
        UniProtSummaryRaf-1:
        FUNCTION:Involvedinthetransductionofmitogenicsignalsfromthecellmembranetothenucleus.PartoftheRas-dependentsignalingpathwayfromreceptorstothenucleus.ProtectscellsfromapoptosismediatedbySTK3.
        CATALYTICACTIVITY:ATP+aprotein=ADP+aphosphoprotein.
        COFACTOR:Binds2zincionspersubunit.
        SUBUNITSTRUCTURE:InteractswithRasproteins.TheinteractionwithRasisantagonizedbyRIN1.WeaklyinteractswithRIT1Bysimilarity.InteractswithSTK3,whichinhibitsitspro-apoptoticactivity.
        TISSUESPECIFICITY:Inskeletalmuscle,isoform1ismoreabundantthanisoform2.
        POST-TRANSLATIONALMODIFICATION:PhosphorylateduponDNAdamage,probablybyATMorATR.PhosphorylationatThr-269increasesitskinaseactivity.
        INVOLVEMENTINDISEASE:DefectsinRAF1arethecauseofNoonansyndrometype5(NS5)[MIM:611553].Noonansyndrome(NS)isadisordercharacterizedbydysmorphicfacialfeatures,shortstature,hypertelorism,cardiacanomalies,deafness,motordelay,andableedingdiathesis.Itisageneticallyheterogeneousandrelativelycommonsyndrome,withanestimatedincidenceof1in1000-2500livebirths.Ref.24Ref.25
        DefectsinRAF1arethecauseofLEOPARDsyndrometype2(LEOPARDsyndrome-2)[MIM:611554].LEOPARDsyndromeisanautosomaldominantdisorderallelicwithNoonansyndrome.TheacronymLEOPARDstandsforlentigines,electrocardiographicconductionabnormalities,ocularhypertelorism,pulmonicstenosis,abnormalitiesofgenitalia,retardationofgrowth,anddeafness.

        MEK1:
        FUNCTION:CatalyzestheconcomitantphosphorylationofathreonineandatyrosineresidueinaThr-Glu-TyrsequencelocatedinMAPkinases.ActivatesERK1andERK2MAPkinases.
        CATALYTICACTIVITY:ATP+aprotein=ADP+aphosphoprotein.
        ENZYMEREGULATION:Activatedbyphosphorylation.
        SUBUNIT:InteractswithMORG1(Bysimilarity).InteractswithYersiniayopJ.
        PTM:PhosphorylationonSer/ThrbyMAPkinasekinasekinases(RAForMEKK1)regulatespositivelythekinaseactivity.
        PTM:AcetylationbyYersiniayopJpreventsphosphorylationandactivation,thusblockingtheMAPKsignalingpathway.
        DISEASE:DefectsinMAP2K1areacauseofcardiofaciocutaneoussyndrome(CFCsyndrome)[MIM:115150];alsoknownascardio-facio-cutaneoussyndrome.CFCsyndromeischaracterizedbyadistinctivefacialappearance,heartdefectsandmentalretardation.Heartdefectsincludepulmonicstenosis,atrialseptaldefectsandhypertrophiccardiomyopathy.Someaffectedindividualspresentwithectodermalabnormalitiessuchassparse,friablehair,hyperkeratoticskinlesionsandageneralizedichthyosis-likecondition.TypicalfacialfeaturesaresimilartoNoonansyndrome.Theyincludehighforeheadwithbitemporalconstriction,hypoplasticsupraorbitalridges,downslantingpalpebralfissures,adepressednasalbridge,andposteriorlyangulatedearswithprominenthelices.TheinheritanceofCFCsyndromeisautosomaldominant.
        SIMILARITY:Belongstotheproteinkinasesuperfamily.STESer/Thrproteinkinasefamily.MAPkinasekinasesubfamily.
        SIMILARITY:Contains1proteinkinasedomain.

        Erk2:
        FUNCTION:CatalyzestheconcomitantphosphorylationofathreonineandatyrosineresidueinaThr-Glu-TyrsequencelocatedinMAPkinases.ActivatesERK1andERK2MAPkinases.
        CATALYTICACTIVITY:ATP+aprotein=ADP+aphosphoprotein.
        ENZYMEREGULATION:ActivatedbyphosphorylationontyrosineandthreonineinresponsetoinsulinandNGF.Bothphosphorylationsarerequiredforactivity
        SUBUNITSTRUCTURE:InteractswithMORG1Bysimilarity.BindstoHIV-1NefthroughitsSH3domain.Thisinteractioninhibitsitstyrosine-kinaseactivity.InteractswithitssubstratesHSF4andARHGEF2.InteractswithNISCH.
        DOMAIN:TheTXYmotifcontainsthethreonineandtyrosineresidueswhosephosphorylationactivatestheMAPkinases.
        PTM:DuallyphosphorylatedonThr-185andTyr-187,whichactivatestheenzyme.
        MolecularWeight05-797RSP:42and44kDa;05-747SP:45kDa;05-538SP:74kDa
        PhysicochemicalInformation
        Dimensions
        MaterialsInformation
        MaterialsInformation
      售后保障
      螞蟻淘生物188，秉承螞蟻淘一貫的嚴謹態度，由螞蟻淘公司專業人員負責品控、采購、物流、銷售、售后，保障正品優質。以“快速好省，為科研提供好產品、好價格”為理念，直接鏈接原廠家，從全國各地原制造商嚴格挑選188款科研精品，剔除品牌溢價，188生物新電商，把好的產品帶給科研！? 力求給你最優質的商品。
    • Q：生物188產品正品保障嗎？
      A：生物188質量把控人員具有十年的從業經驗，在業界享有良好的口碑；自營商城，直接從廠家采購， 自己的團隊負責國際物流和清關，中間沒有第三方，所有流程嚴格把控，100%保證正品，假一罰十。

      Q：下單后可以修改訂單嗎？
      A：下單后的商品付款之前可以修改；訂單付款成功，需要聯系我們客服進行修改；客服電話：4000-520-616

      Q：可以開發票嗎？
      A：本網站所售商品都是正規清關，均開具16%正規發票，發票金額含配送費金額，另有說明的除外。

      Q：商品幾天可以發貨？
      A：生物188商品，全部現貨銷售，付款后即可發貨,一般一周內送達！

      Q：如何聯系商家？
      A：有任何問題夠可以電話咨詢我們，全國24小時免費服務熱線：4000-520-616 或聯系我們的在線客服QQ：1570468124

      Q：收到的商品少了/發錯了怎么辦？
      A：同個訂單購買多個商品可能會分為一個以上包裹發出，可能不會同時送達，建議查看訂單詳情是否是 部分發貨狀態；如未收到，可聯系在線客服或者致電4000-520-616。

      Q：退換貨/維修需要多長時間？
      A：一般情況下，退貨處理周期為客戶收到產品一個月內（以快遞公司顯示簽收時間為準），包裝規格、 數量、品種不符，外觀毀損、短缺或缺陷，請在收到貨24小時內申請退換貨；特殊商品以合同條款為準。

    客戶服務

    在線客服
    用戶反饋

    何為188

    極簡而嚴謹，我們僅銷售188款生物醫學科研用品，款款都是爆款；因為少所以聚焦，聚焦甄選每一款產品，聚焦服務每一位客戶!

    關注我們 :

    點擊QQ聯系我們
    生物188微信

    關注188微信公眾號

    獲取最新優惠活動通知

    • 品質甄選，正品保證

    • 自營電商，廠家直采

    • 極簡主義，188精品

    常見問題 | 購買相關 | 配送與驗收 | 支付問題 | 售后與服務 | 服務協議 | 隱私政策 | 何為188 | 中國智造 | 聯系我們
    友情鏈接： ImmunoChem中國 | Paragon Genomics價格 | pingfanwangba產品代理 | Matreya官網 | ezbiosystems代理 | 螞蟻淘 | 組織因子

    公司版權所有 ? 2005-2021 蘇州螞蟻淘生物科技有限公司 蘇ICP備17049038號-10

  • <menu id="uuusw"></menu>
    •
  • <table id="uuusw"></table>
    上床视频